FastQCFastQC Report
Tue 25 Mar 2025
SH_dif_16_S5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH_dif_16_S5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41206632
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7676121.862836059981801No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT28200.017.2554231
CGGATAG77100.016.199983
TCGGATA85200.015.6611222
CGACGCA75550.014.8391121
TTGATCG133000.014.5452077
GCGGAAT82400.014.4739591
CTCGGAT125400.014.1901131
ATAGCCG87000.013.4765556
TGATCGC132900.013.3533798
CGATTGT50200.012.7740743
CGGGAAT79700.012.74957751
GGGGAAT265000.012.7276451
TAGCCGG92350.012.6829257
GGGAAAT333850.012.6606751
CGACTAA67850.012.48876928-29
CCGGGAT123250.011.4959291
CGGCAAT46300.010.921931
ACCCGAC69500.010.7416726
CGGGGTT126250.010.6181651
CGGGGAT104450.010.4592741
Produced by FastQC (version 0.11.4)