FastQCFastQC Report
Tue 25 Mar 2025
SH_dif_15_S3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH_dif_15_S3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49057702
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40180678.190491678554368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCG201350.017.9769577
CGCGATT28250.017.7332761
TGATCGC204300.016.6273088
CGGGGTT92300.013.8015461
GGGGAAT255250.013.495521
GATTGAT333650.012.9097445
CGCCGTT152850.012.53664912-13
GATCGCC280600.012.3535879
CGGGAAT95300.012.0153521
CGACTAA83300.011.97547128-29
ATTGATC361950.011.3582946
AGATTGA530900.011.1962924
GGGAAAT383000.011.0619671
TCGCCGT178800.010.81000510-11
CGCGGTT54450.010.7776821
CGAGTAG129250.010.5942331
CGGGGAT104300.010.5668571
CGGGATT81500.010.30322551
GCCGTTC188100.010.14945312-13
CCGTTCT197200.09.93963914-15
Produced by FastQC (version 0.11.4)