FastQCFastQC Report
Tue 25 Mar 2025
SH_dif_14_S6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH_dif_14_S6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39171657
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36282279.262378152652568No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG515160.13151345627273311No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG102550.019.9693153
TACCGAT152800.017.6295285
GTTAGTT155400.017.4934641
CACCCGG121300.017.3953364
ACCCGGC134400.016.2338395
ACCGATT166800.016.1498326
ACTACCG171700.015.967593
CTACCGA192850.014.3156564
CGACTAA63250.014.15638728-29
CCGATTG195050.013.8295837
CGCAGTT414250.013.7695781
CGATTGG198800.013.5704658
TTATCCG420450.013.5685328
CGCTATT74700.013.4714331
TATCCGG426800.013.374169
TGCGCTC131500.012.9827029
ACGGACA134800.012.8509932
TTGATCG107250.012.709837
CGCGATT24300.012.5610431
AGTTTTA685450.012.4111214
Produced by FastQC (version 0.11.4)