FastQCFastQC Report
Tue 25 Mar 2025
SH_dif_13_S4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH_dif_13_S4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44625137
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43644599.780270254408405No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT28400.018.1420571
CGAGTAG168050.017.0614931
TTGATCG160200.015.462127
CGGGGTT91100.014.1392551
TGCGCTC203650.014.1187629
TGATCGC171450.013.7365168
CGACTAA74250.013.56586128-29
GGGGAAT223150.012.955581
GAGTAGC225950.012.5760612
TGATTCG112850.012.4252973
GGGAAAT334350.012.4136371
CGGGGAT101100.011.8913331
CGGGAAT92050.011.8165911
GATTGAT259000.011.7701625
CGTAGGG118200.011.72307464-65
CGATTGT50750.011.5987133
CGCCGTT118350.011.44189912-13
TAGCTGC260250.011.1803535
AACGTAG124050.011.11241862-63
ACGTAGG132750.011.105268562-63
Produced by FastQC (version 0.11.4)