FastQCFastQC Report
Tue 25 Mar 2025
SH-9_S2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-9_S2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20984722
Sequences flagged as poor quality0
Sequence length101
%GC59

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1923570.9166526008779149No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA290530.13844834351391455No Hit
CGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC215470.1026794636593232No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG265800.025.2262231
CGAAGCG291350.023.1439343
GCGAAGC318600.022.0407052
GAAGCGG335000.020.9193214
TCGGATA140650.020.3877942
TGCGACA59100.020.0966476
GCGACAT59550.019.9447827
CGGATAG141550.019.923873
CGATCTG44250.019.7831654
GGCGATC49550.019.7680592
AAGCGGG358100.019.5038075
GCGATCT51350.019.3515683
CTCGGAT163650.018.9103281
ATAGCCG158350.017.9594786
CTCGGTT112850.017.8626292
TCGGTTG112250.017.6630233
CCTCGGT125300.017.3340051
AGCCGGT167200.017.1220768
TAGCCGG171050.016.6537027
GATAGCC173400.016.6190245
Produced by FastQC (version 0.11.4)