FastQCFastQC Report
Tue 25 Mar 2025
SH-9_S2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-9_S2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20984722
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG753220.3589373259269291No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG606200.2888768314395588No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT423660.20188973673322905No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC391580.18660242437331312No Hit
GCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAG370060.1763473445109256No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG352190.16783162531293006No Hit
CCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG328180.1563899679014094No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC318220.15164365770487692No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT293300.13976835147017913No Hit
CGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGG284630.13563677422078788No Hit
GTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCCAC279910.13338751878628652No Hit
CTGTAACACTCGGGGGGGGTTTCGGTCCCGCCGCCGCCGCCGCCGCCGCC239620.11418783627440954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAT45950.030.3933871
GAGTCTT161950.029.5739044
TAACACG85850.028.1636962
AGTCTTG173900.027.7329185
TGGAGTC179000.027.413182
AACACGT89700.027.006043
GGAGTCT181150.026.5616023
AGAAGCG188750.025.7524054
GCTACGT46300.025.7499223
GCCAGAA203100.025.2130951
CGGTATT53550.025.1924862
GTCTTGG191600.025.170896
TGCTACG47450.024.9273032
ACACGTG98150.024.786134
GAAGCGA194650.024.6789045
CTCGCTA20850.024.377831
TCTTGGA200400.024.2078427
ACGTACG48800.024.0494526
AAGCGAG202150.023.8102386
CTTGGAA199150.023.4531568
Produced by FastQC (version 0.11.4)