FastQCFastQC Report
Tue 25 Mar 2025
SH-8_S1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-8_S1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37087243
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6389531.7228376884202474No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC434930.11727213047354317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG99650.018.8986321
CGCGTGC221750.018.5643711
TGATTCG98150.017.1626473
CGAAGCG110100.016.847033
GCGTGCC242100.016.5739362
CGCGGTG197350.016.4479431
CGGGGAG410350.015.9474911
ATTCGGC119100.015.4944965
GCGAAGC134450.014.0774442
TTCGGCT129500.013.7020686
GATTCGG141800.013.6480844
GGCGCGT176950.013.0227597
CGTGCCT312200.012.9889063
AGCGGTC87500.012.8163666
GAGCGGT107650.012.5272415
GGGGAAT258350.012.5094111
CGGGGAT117950.012.3559871
TGGCGCG208500.012.096116
GGGAGAG591400.011.79344751
TTGAGTC454500.011.6706862
Produced by FastQC (version 0.11.4)