FastQCFastQC Report
Tue 25 Mar 2025
SH-8_S1_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-8_S1_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37087243
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGGGG953580.257118060784405TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGGTT751120.20252786113003873TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTGGGTT483910.1304788279894518TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTTT480400.12953241091552692TruSeq Adapter, Index 7 (97% over 35bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG441070.11892768626667666No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG397430.10716083694870498No Hit
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC386850.10430810400223064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCT724200.028.8783538-39
TCGTATC760500.028.75532338-39
TTCGTAT820550.027.87523336-37
ATTCGTA819750.027.71991536-37
AATTCGT874900.026.5561934-35
TATCTCG388050.026.21274840-41
GAATTCG895850.026.05180534-35
CGGGATT213600.025.8419761
CGAATTC913000.025.84077632-33
TTCGGCG207400.025.6357386
ACGAATT932900.025.52885832-33
CACGAAT959800.024.85543630-31
GGATTCG218200.024.7943633
GTCACGA1015650.024.1620928-29
TCGGCGA223050.023.9222497
AGTCACG1066350.023.85516428-29
ATTCGGC234350.023.6203335
GAGCACA2159700.023.4238519
TCACGAA1032250.023.22595830-31
AGAGCAC2217050.022.736498
Produced by FastQC (version 0.11.4)