FastQCFastQC Report
Tue 25 Mar 2025
SH-7_S15_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-7_S15_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26170013
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3599721.3755132639788905No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC615760.23529220256787797No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC517930.19790972209299246No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA393560.15038586339257837No Hit
CGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTT353080.1349177778398505No Hit
GGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCAC274570.1049177927424033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG211900.024.764721
CGAAGCG229800.022.8768843
CGCGGTG202750.022.4515881
GCGAAGC236650.022.4346472
AGCGGTC124950.021.6995756
GAGCGGT139450.020.5969565
CGATCGA45600.020.0274354
GCGGAAT55900.019.2152881
TCGGATA59700.019.0225942
AAGCGGG287450.018.8484675
CTCGGAT76250.018.803371
CGGAATC50500.018.7400822
CGGATAG59700.018.547033
CTTGAGC437300.018.4167481
TGGCGCG218700.018.3692656
GAAGCGG309050.018.2966674
CGGGGAG529000.017.979321
TTGAGCC445200.017.909262
ATCAGCG54900.017.4967176
GATTCAA234500.017.2526635
Produced by FastQC (version 0.11.4)