FastQCFastQC Report
Tue 25 Mar 2025
SH-7_S15_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-7_S15_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26170013
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG951220.3634770834848267No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA800740.305976156756208No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG731640.2795718901629892No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG654050.24992345246446762No Hit
CGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGG586660.22417260549316503No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG568810.2173518217205318No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC528240.20184934566138732No Hit
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC403380.15413824975937154No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG383270.14645388215894276No Hit
CGGGATTCGGCGAGTGCTGCTGCCGGGGGGGGCTGTAACACTCGGGGGGG362790.1386281313654678No Hit
CGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGGGGG337240.12886504871052223No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT314190.12005725790048329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA44100.030.3756391
TCGCTAT44850.030.2911682
CCCGGAT197450.029.158181
CCGGATT291100.026.370241
CGGATTT356400.025.7504221
CTGGAGT436100.024.3665921
GACGGGT298450.023.5083072
ACGGGTC300750.023.1374443
CGACGGG329400.022.2658181
GGAGTGC340500.022.2637943
CGGGATT769150.022.1593131
AAGCGAG177050.022.0606675
GTGCAGT343750.021.8954736
GAGTGCA350150.021.834614
GAAGCGA178700.021.6974744
ATTCGGC808100.021.5266955
ACGAGAT187100.021.4771932-33
TTCGGCG807800.021.4522976
AGTCACG212100.021.40908628-29
AGTGCAG358300.021.4042225
Produced by FastQC (version 0.11.4)