FastQCFastQC Report
Tue 25 Mar 2025
SH-6_S14_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-6_S14_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23354574
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1981710.8485318550447549No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC771420.3303078874399507No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC732250.3135360122603821No Hit
CGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGA255670.10947320212306164No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT242270.10373556803048517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTG161550.028.266841
CGCGAAG80550.022.7353841
GGCGCGT193150.022.6867947
TGGCGCG195250.022.6124426
CGAAGCG90200.019.9358123
GCGCGTG223800.019.7912248
GTGGCGC231900.019.2631865
CTTGAGC450000.018.802341
CGCGTGC249650.018.1589531
GCGAAGC100400.018.051862
TTGAGCC460200.017.9742872
AGCGGTC78400.017.6247776
CGGGGAG332550.017.531161
CGGTGGC263100.017.2846033
GCGTGCC262500.016.9275132
GACCACC437800.016.47820794-95
GAGCGGT86800.016.464335
GGGACCA442650.016.41527292-93
TGAGCCC502150.016.3407693
GGACCAC424850.016.20638592-93
Produced by FastQC (version 0.11.4)