FastQCFastQC Report
Tue 25 Mar 2025
SH-4_S1_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-4_S1_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58300540
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5172830.8872696547922199No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT1226610.21039427765163066No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1063970.18249745199615647No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1061290.1820377650018336No Hit
CGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC963450.16525575920909136No Hit
GGAGGATTCAACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGAT763680.1309902103822709No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA603310.10348274647198807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG540700.019.0014821
TTGACTC729200.018.24842
CGCGGTG245950.018.2457351
TCTAGTC708000.017.994227
CTTGACT774150.017.8417261
CTCTAGT733750.017.4786036
TAGTCTG731750.017.3071239
CGAAGCG604800.016.8157753
CTCGCTA88300.016.7978421
CTAGTCT754000.016.7582428
CTTGAGC761400.016.5150451
GCGAAGC688750.016.3161412
TTGAGCC758950.016.1388992
GACTCTA810050.016.0946624
TCGCTAT94600.015.9288412
GCGACAT164700.015.8604997
ACTCTAG820300.015.8187315
AACGGGT106800.014.8169282
GGCGATC129500.014.5906552
TGCGACA179100.014.3478986
Produced by FastQC (version 0.11.4)