FastQCFastQC Report
Tue 25 Mar 2025
SH-3_S6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-3_S6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16587552
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1570340.946697861143103No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1477140.8905111495656503No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC883260.5324836359216839No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA397730.2397761887950675No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT286060.17245462139319895No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT232270.14002668989372272No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT230510.1389656532802429No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT229540.1383808774194046No Hit
CGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGA227260.13700635271557854No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT220690.13304555126639542No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG186340.1123372514521733No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG178260.10746612881756151No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTG177880.10723704136692382No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT166820.10056939082994283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTG164950.033.877221
CTCGCTA53300.031.3370231
CTTGAGC622250.027.838451
TCGCTAT62850.027.4788042
TTGAGCC634450.027.049572
TGGCGCG196100.025.4315746
TGAGCCC686750.024.9826983
CGGTGGC214450.024.6234193
GAGCCCA717150.024.2271824
GGCGCGT195400.023.9244867
GTGGCGC220000.023.0559145
CAGGAGT1184750.022.4929359
CCCAGGA787750.022.1039377
CCAGGAG1257150.021.491138
AGCCCAG819700.021.3289625
TTGAGTC478800.020.7725032
GCGCGTG223300.020.744568
CTTGAGT487750.020.5077441
TGATTCG83800.020.1573963
CGCGTGC252350.020.0815121
Produced by FastQC (version 0.11.4)