FastQCFastQC Report
Tue 25 Mar 2025
SH-2_S16_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-2_S16_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35760665
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2788740.7798344913328653No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC765990.21419903684677005No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC735430.2056533344667947No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAGC525200.019.6575811
TTGAGCC527250.018.8901842
CGCGGTG167950.017.7202871
CTTGAGT521950.017.359611
TTGAGTC494250.017.155052
CGCGTGC187200.016.9090961
CAGGAGT1107350.016.6426569
TGAGTCC510750.016.5823213
TGAGCCC596550.016.4340133
GAGTCCA539300.016.4063474
CTCGCTA47250.015.8217811
GAGCCCA647500.015.5719994
GGCGCGT151450.015.4957687
CCAGGAG1268200.015.3899388
AGTCCAG588150.015.2045795
GTCCAGG624100.014.35907656
AGCGGGG517050.014.26460486-87
CGGGGGA472400.014.23267188-89
TCGCTAT56250.014.21559052
GCGTGCC219750.014.2107112
Produced by FastQC (version 0.11.4)