FastQCFastQC Report
Tue 25 Mar 2025
SH-2_S16_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-2_S16_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35760665
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG841170.23522213582996848No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTGGGGG488430.1365830305448738TruSeq Adapter, Index 3 (97% over 36bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG433970.12135400725909319No Hit
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC410040.1146622972475484No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA72700.033.3241541
TCGCTAT74850.031.6046642
GCGCATC384350.024.19728538-39
TGCGCAT407000.023.95937236-37
ATGCGCA447550.023.40172836-37
CGCATCT374550.022.54138838-39
TAATGCG466250.022.3969334-35
AATGCGC468950.022.04514534-35
CTGGAGT674050.020.7899421
GGAGTGC517800.018.6951033
CTAATGC558650.018.60323132-33
GTGCAGT524200.018.3102786
GAGTGCA548150.017.9437054
AGTGCAG548500.017.8975775
ACTAATG610200.017.63488832-33
CGCTATG136800.017.2219093
CGCATCG50900.016.7038338-39
TGGAGTG623350.016.406872
CACTAAT705650.015.37739230-31
CAGTGGC669700.015.1977229
Produced by FastQC (version 0.11.4)