FastQCFastQC Report
Tue 25 Mar 2025
SH-1_S5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-1_S5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39749822
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2593350.6524180158592912No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1296210.32609202627372774No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1091030.274474185066791No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG951770.23944006592029518No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTG602410.15155036417521567No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT468360.11782694272190702No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA462540.11636278522203194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTG299550.024.766131
CGTGCCT743350.024.2946241
GGCGATC136750.020.2053762
CCTGTAG932650.019.9621565
GCGATCT129500.019.911693
GGGCGAT140750.019.4630781
TAGTCCC953200.019.3233459
GTAGTCC959300.019.2054028
TGTAGTC990450.018.8784487
GCCTGTA1022650.018.8576934
TGGCGCG372000.018.1748436
GTGCCTG1039700.017.884052
TGCCTGT1055450.017.7471753
GGCGCGT362150.017.4150057
CGCGTGC494200.017.1464521
CGGTGGC425150.016.9599343
GTGGCGC427400.016.1289625
CGATCTG157900.015.4014784
GCGTGCC573700.014.6384442
CTTGAGC931900.014.1599121
Produced by FastQC (version 0.11.4)