FastQCFastQC Report
Tue 25 Mar 2025
SH-18_S4_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-18_S4_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25646691
Sequences flagged as poor quality0
Sequence length101
%GC63

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2878461.122351417576638No Hit
TGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCC614860.23974242915002172No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT603960.23549236819673927No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA479120.18681552329694306No Hit
AGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGA402490.15693642505382077No Hit
CTCGGATAGCCGGTCCCCCGCCTGTCCCCGCCGGCGGGCCGCCCCCCCCT371620.14489978453750624No Hit
GGGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGA332290.1295644728592862No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCCGCCTGTCCCCGCCGGCGGGC319730.12466715491678829No Hit
CCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTG318610.1242304514059923No Hit
GGTGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCC306660.11957098091133862No Hit
GAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCC304250.1186312885354294No Hit
GGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGG298710.11647116581238491No Hit
GGAAAGAAGACCCTGTTGAGCTTGACTCTAGTCTGGCACGGTGAAGAGAC295820.11534431478899168No Hit
CGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC276930.10797884218279855No Hit
GGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAGTGGCGGA271140.10572124099752284No Hit
TGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGG260850.10170902749208466No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG296300.029.1780321
CGAAGCG315150.026.9373073
GCGAAGC348250.026.279342
TGCGACA76200.025.5232496
GAAGCGG354700.024.7075044
GCGACAT77800.024.694237
GCTGCGA91450.021.629264
CAGTCGA145500.021.5300034
AAGCGGG413250.021.4015645
CTGCGAC93050.021.3590535
TCGGATA379150.021.2171462
GATCGTT81100.021.0637365
TGATCGT87350.020.640074
CGGATAG385300.020.4609173
AGTCGAG153700.020.3505745
CTCGGAT418100.019.9309621
ATAGCCG398150.019.907526
CGACATC97800.019.7410568
ACGTGCG45500.019.5521956
ATCCTAG78900.019.3120296
Produced by FastQC (version 0.11.4)