FastQCFastQC Report
Tue 25 Mar 2025
SH-18_S4_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-18_S4_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25646691
Sequences flagged as poor quality0
Sequence length101
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG928250.36193753026462555No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG877400.3421104110467896No Hit
CCCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGC855460.3335557012013753No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC851470.33199994494416457No Hit
CCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAGT759290.2960576863502586No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA712720.2778993984058216No Hit
CCCAGGACGAAGGGCACTCCGCACCGGACCCCGGTCCCGGCGCGCGGCGG667420.2602363010495194No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG573120.22346742509589249No Hit
CGACGGGTCTCGCTCCCTCGGCCCCGGGATTCGGCGAGTGCTGCTGCCGG471240.18374300216741413No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT447000.17429149046947226No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGC413420.16119818342257097No Hit
GCCAGAAGCGAGAGCCCCTCGGGGCTCGCCCCCCCGCCTCACCGGGTCAG413200.16111240237580748No Hit
CCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACC382100.14898608167424016No Hit
CACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCC368600.14372224471375275No Hit
CCCCAGTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGGGGGGGGGG362180.14121899780365427No Hit
CGACGCTTTCCAAGGCACGGGCCCCTCTCTCGGGGCGAACCCATTCCAGG340810.13288653885212717No Hit
CCCGGCGCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAG334950.1306016436974267No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG309850.12081480608940934No Hit
CCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG301260.11746544612714364No Hit
CCCGGGGCTCCCGCCGGCTTCTCCGGGATCGGTCGCGTTACCGCACTGGA294830.11495830007855594No Hit
CTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAGA286300.11163233494722574No Hit
CGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCG274020.10684419288242682No Hit
CCCCGAGCCACCTTCCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGGG270170.1053430245640656No Hit
GCTCGCTCCGCCGTCCCCCTCTTCGGGGGACGCGCGCGTGGCCCCGAGAG266520.10391983901548937No Hit
CTCGCCTTAGGACACCTGCGTTACCGTTTGACAGGTGTACCGCCCCAGTC266430.10388474676908611No Hit
GTCAAACTCCCCACCTGGCACTGTCCCCGGAGCGGGGGGGGGGGGGGGGG258080.10062896613056242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACGT70300.038.7832373
TGCTACG71900.037.6593132
ACGTACG79950.034.529216
CTACGTA82500.033.2893524
CGTACGA85550.032.4910667
CTCGCTA63500.032.4674031
TAACACG109500.032.406562
TACGTAC87100.031.640295
CCGGTAT78050.031.5882781
GAGTCTT166150.030.856924
AACACGT119000.030.2957923
TGGAGTC170900.030.2699892
AGAAGCG324450.029.9533483
GGAGTCT173850.029.7536773
GAAGCGA326550.029.581434
AGTCTTG178550.029.2727455
ACACGTG123100.029.2578624
CTAACAC130100.028.0059031
CGGTATT89750.027.3112562
AAGCGAG359350.026.9209735
Produced by FastQC (version 0.11.4)