FastQCFastQC Report
Tue 25 Mar 2025
SH-16_S12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-16_S12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39147017
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1605320.41007466801365733No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC726870.18567698274430464No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC651340.16638304778113744No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCT333800.023.9850861
TAGTCCC413700.019.627239
GTAGTCC417450.019.5642668
TGTAGTC447950.018.4856937
CCTGTAG446200.018.2824735
CGCGTGC220750.018.0698321
CTTGAGC543450.017.5968111
CGCGGTG204900.017.1582951
GCCTGTA522050.016.8497924
TCAAACG97950.016.5214798
TTGAGCC582050.016.1940782
GGCGCGT171950.016.0157787
CTTGAGT538900.015.5239161
TTGAGTC529900.015.314312
CAAACGA110450.014.8230519
GCGTGCC264650.014.6611882
TGGCGCG204500.014.3689666
TAGACCG92650.014.2490548
CAGGAGT1221000.014.0869189
TGCCTGT609600.014.0029263
Produced by FastQC (version 0.11.4)