FastQCFastQC Report
Tue 25 Mar 2025
SH-15_S11_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-15_S11_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23196090
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2112770.910830230439699No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG756210.3260075297172929No Hit
GGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTC636620.27445142694307534No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC584360.25192176784966774No Hit
TATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCG581900.2508612442872915No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG551340.23768661011403214No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC524300.22602947307067697No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC515290.2221451977466892No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA488240.21048374963194227No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG440590.18994149445014225No Hit
CGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAAT422520.18215138844520778No Hit
GGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATT421240.18159957130706078No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA415100.17895257347251198No Hit
CGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGG399180.1720893478168088No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA387330.16698072821755736No Hit
GGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTA385550.1662133575098217No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA340000.14657642732029405No Hit
TTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCG327860.1413427866506812No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG325230.14020897487464482No Hit
CGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT293560.12655581177689862No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG283660.12228785109904299No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA279030.12029182504465193No Hit
GTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTG273660.11797677970726962No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTG263010.113385488675031No Hit
AAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGT257740.11111355405156645No Hit
CTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT255900.11032031691548015No Hit
GGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTAT255800.11027720620156241No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAG248360.10706976908608304No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT245860.10599200123813972No Hit
TGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCA245090.10566004874097315No Hit
GGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCG241570.10414255161106892No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT241480.10410375196854298No Hit
CGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGC241040.10391406482730495No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAAC236730.10205599305745063No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTCAA324150.029.1967755
ATATTCA329250.028.8020114
AATATTC331200.028.5609993
GTTGACG125050.028.4567346
CAAACGA353950.026.5514499
TTGACGC136200.026.1966157
ACCCGTT122650.026.0427686
TAACCCG123000.025.5454714
CGCAAAT107050.025.3732531
CCTGTAG336550.025.2245675
GGCGATC76950.025.1515982
TTCAAAC375250.025.0821657
AACCCGT128500.025.0781155
CCGTTGA127700.024.9017168
ATTCAAA386850.024.5502366
CAAATAT411700.024.4476741
CGTTGAA131900.024.0370379
GGTAACC135800.023.9391042
ATCCGAC40000.023.896946
GCCTGTA370000.023.8649674
Produced by FastQC (version 0.11.4)