FastQCFastQC Report
Tue 25 Mar 2025
SH-14_S10_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-14_S10_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21999743
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8751683.9780828348767527No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC926430.42110946477874767No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC831830.3781089624546978No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG407810.18537034728087504No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA307040.1395652667397069No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT281790.12808785993545468No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTG251380.11426497118625432No Hit
CGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGA235600.10709216012205233No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTG157750.027.3554551
TCAAACG68900.023.2808558
GGCGATC71250.022.7122252
GCGATCT70800.022.3219263
CAAACGA73950.021.946969
GGGCGAT79650.021.6239011
GGCGCGT189550.021.4680757
TGGCGCG193300.021.369826
CGTGCCT356800.019.693371
CTTGAGC478850.019.110651
CGGTGGC228000.018.8230883
CGATCTG83400.018.5524024
CGCGTGC268350.018.4084551
TATTCAA92250.018.2600545
GTGGCGC233100.018.0864735
TAGACCG65900.018.0221588
TTGAGCC504050.017.9486892
TAGTCCC408850.017.2093899
CCTGTAG413300.017.1729265
GTAGTCC410200.017.129688
Produced by FastQC (version 0.11.4)