FastQCFastQC Report
Tue 25 Mar 2025
SH-13_S9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-13_S9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33715229
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2124490.630127708757369No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC571880.1696206779434896No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC454430.13478478820357412No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCT243750.023.3327621
TAGTCCC303700.018.8048329
GTAGTCC315500.018.1914948
CCTGTAG336350.017.1481765
CGCGTGC166800.017.1051791
TGTAGTC349500.016.7873237
CTTGAGC462300.016.130161
GCCTGTA406650.015.7311914
TTGAGCC481650.014.9221882
TTGAGTC383500.014.8301462
CTTGAGT402800.014.6129281
CGCGGTG173100.014.2685481
GGCGCGT135300.014.2332347
TGAGTCC424550.013.7194133
GCGTGCC201350.013.7000852
GAGTCCA459050.013.6881184
CAGGAGT961750.013.0915079
TGCCTGT478950.012.9119473
TGAGCCC569350.012.6319553
GTGCCTG473000.012.4241522
Produced by FastQC (version 0.11.4)