FastQCFastQC Report
Tue 25 Mar 2025
SH-12_S8_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-12_S8_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43060074
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG4114690.9555696536889371No Hit
CGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATA2245470.5214737903144337No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG2244590.5212694246647138No Hit
CGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGG2177410.505667965178137No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC2162590.5022262618498984No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA2046430.4752499960868623No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC1864790.4330670681151175No Hit
TGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGC1773450.411854842608956No Hit
CCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCC1531540.3556751899683219No Hit
ATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCC1505200.34955815449829464No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA1451000.3369710883450874No Hit
GGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGA1372930.3188406039432259No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT1363390.3166250945133071No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1356180.31495068958776057No Hit
CCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCA1298230.3014927470863148No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC1242940.2886525462078862No Hit
CTCGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACAT1242180.2884760486013099No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA1239370.28782347192436314No Hit
GGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATG1211900.2814440123814No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGG1204600.2797487064234957No Hit
CCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGT1198290.2782833118215264No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1192120.27685042993655795No Hit
CCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTT1144410.26577056045003544No Hit
GTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTT1108990.25754484304880665No Hit
GGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTC1098660.25514586900152564No Hit
GGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGCA1072720.2491217270086438No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG1046490.2430302372448315No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1038950.24127919520063992No Hit
TTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCAT1037660.24097961373684587No Hit
GTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCA1033550.2400251332591765No Hit
GCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGC1013840.23544780717283487No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC1000940.2324519925348944No Hit
CTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCCAAGCC989290.22974647001303342No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC974080.22621419554457803No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG923570.21448407171803743No Hit
CAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCAT874040.2029815369105032No Hit
GCTGATTCCGCCAAGCCCGTTCCCTTGGCTGTGGTTTCGCTGGATAGTAG865150.20091697938094577No Hit
CTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTG840940.19529460167671797No Hit
GAACAATCCAACGCTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG837260.1944399816869799No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC825820.1917832282406203No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC805600.18708746297091827No Hit
GGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGC780030.18114924744439595No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG777180.18048738141973467No Hit
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC774270.1798115813735016No Hit
CGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGG764340.17750550080336602No Hit
CGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAG756000.17556867180488356No Hit
TGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGG751990.17463741469650051No Hit
GCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGA745010.17301642352031257No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG736330.1710006350662565No Hit
ACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTC733710.17039218279095386No Hit
GGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGCAT731800.16994861643758438No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT731460.16986965698201076No Hit
CTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTC729890.1695050500842149No Hit
CTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTAC723040.16791424928809923No Hit
GAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGA722950.1678933482557415No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGAT707390.16427979199478385No Hit
CTAGAATTACCACAGTTATCCAAGTAGGAGAGGAGCGAGCGACCAAAGGA700120.16259145304766545No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCT676440.15709215920065533No Hit
CACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTAC671060.15584274193304914No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC663990.15420084972450349No Hit
CGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCA663570.15410331157350077No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA661650.15365742288320267No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA655260.15217344958580425No Hit
GTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCAT650060.15096583438291353No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCG635870.14767043828117898No Hit
GGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCC630430.146407086992001No Hit
CCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGG613680.14251717263653563No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT601460.1396792769097424No Hit
CGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTT598730.13904527892822477No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC588480.13666488357637285No Hit
CGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCG581880.13513214120347308No Hit
GGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTA578500.1343471913215941No Hit
TGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGT578470.1343402243108082No Hit
AATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCC567660.13182977809095264No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGG559360.12990223844018475No Hit
CGCTCGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCAC548210.12731283276475558No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC544400.12642802239494524No Hit
CAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATGGC542510.12598910071543304No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG536910.12468859203539687No Hit
GGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCT533400.12387345177344562No Hit
CGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACG532090.12356922563579431No Hit
TAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTCCCGCC524070.12170671141902821No Hit
CAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTAAAC522940.12144428734609235No Hit
TTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCT518440.12039923572820614No Hit
CTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTTACTC518220.12034814431577613No Hit
CTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATTGAATT502720.1167485220763903No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC494690.11488368552269558No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC487520.11321856994486355No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA478200.11105415192737478No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC474630.1102250776438517No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA470820.10934026727404138No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA467860.10865285554316512No Hit
CGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACC465290.10805601495250565No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACC465170.10802814690936202No Hit
GGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTG464870.10795847680150293No Hit
CTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGCATTTGGC462950.10751258811120483No Hit
ACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGGATTACCATG460570.10695987192218945No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTGGGGG459490.10670905953389676TruSeq Adapter, Index 27 (97% over 39bp)
AAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCT459190.10663938942603768No Hit
GTAAAACTAACCTGTCTCACGACGGTCTAAACCCAGCTCACGTTCCCTAT455440.10576851307779918No Hit
GCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTA454630.10558040378657965No Hit
CTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCC452430.10506948966227973No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC451980.1049649845004911No Hit
CTCTCATGTCTCTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCT448510.10415913358625441No Hit
TCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGC443660.10303280017586593No Hit
CTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCTCGTTCA441470.10252420838849464No Hit
TGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCA435290.10108900416659758No Hit
TTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTT434440.10089160552766352No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG433250.10061524743315584No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT432520.1004457168373654No Hit
CGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCTCCACTTCG432070.10034121167557677No Hit
CGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAG431620.10023670651378815No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG40650.039.389715
CCGGACT37400.038.485281
CGCGTAA857100.036.9063571
CTGGAGT392850.034.7281271
GCGTAAC916450.034.624442
CGTAACT925600.034.192813
TGGAGTC126000.033.025442
GCCGTAT160550.031.866111
GGAGTCT137600.031.1369743
AACTAGT1048700.030.1515186
ACTAGTT1062600.029.7034477
TCTCGAC75700.029.6878455
TAACTAG1078800.029.301515
GTAACTA1102000.029.2711074
CCGTATC177650.028.7714732
CGGCTAC24800.028.7318251
AGTCTTG147200.028.6627965
TATACGC357150.028.2962747
GTGCAGT331550.028.274216
TACGCTA361300.027.9974889
Produced by FastQC (version 0.11.4)