FastQCFastQC Report
Tue 25 Mar 2025
SH-11_S7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-11_S7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26935444
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2926331.086423524334702No Hit
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG1747350.6487177267246829No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1634790.6069289223522731No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1303290.4838568838887527No Hit
GGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTC1278320.47458657076527117No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG1155000.4288030299407724No Hit
TATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCG1142170.4240397893571014No Hit
CTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGC1128270.4188793026764288No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA958550.3558693890473831No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA935820.3474306939213625No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG878870.32628754885198846No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA871950.3237184432526896No Hit
GGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATT814590.30242308238913757No Hit
CGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAAT810420.30087493638493573No Hit
CGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGG808320.3000952945123162No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA806210.2993119400593508No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA804690.29874762784678804No Hit
TTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCG777990.2888350383234819No Hit
CGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT769040.2855122789139841No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTG765660.2842574267571012No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG743660.2760897499963245No Hit
GGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTA697360.2589005029952356No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG627140.23283076380697493No Hit
CTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT611030.22684979686987897No Hit
AAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGT593780.22044559577336093No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC570520.21181013388901254No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAG562670.20889575831755364No Hit
GAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTC547190.20314868394224356No Hit
GGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTAT537120.19941011553401533No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT527610.19587945162515236No Hit
ATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGTGAAC508310.18871417155774378No Hit
CGCGGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGC498490.18506841765816073No Hit
TGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCA496590.1843630273924573No Hit
GTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTG489780.18183476017696237No Hit
GGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCG479950.17818529369703356No Hit
CTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGG476530.17691559121876735No Hit
CTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGA457780.1699545030703782No Hit
CTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAG456040.1693085140902077No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT455900.16925653796536638No Hit
TCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATG453760.16846204577136356No Hit
TCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGA451670.1676861164790898No Hit
CTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGG434700.16138586763225438No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCT412210.1530362744345332No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA411790.15288034606000925No Hit
TGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAA400530.14869998059062994No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATGA399220.14821363256532916No Hit
CTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTCCT392840.14584500630470393No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT392720.14580045534055425No Hit
GCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAG382090.14185398243296082No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA380080.1411077537834535No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT370850.13768104212427312No Hit
CGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGCGGGGAAAGAAG367350.1363816390032405No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGA366050.1358990035582855No Hit
TGAATGTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGT357750.13281756187126523No Hit
GGGAAGCTCATCAGTGGGGCCACGAGCTGAGTGCGTCCTGTCACTCCACT353410.13120630200118477No Hit
TAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTT342470.12714473910287127No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG340740.1265024627030466No Hit
AGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCC340140.12627970788229814No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG333130.12367718905988705No Hit
AGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGT331560.12309431394559527No Hit
GGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGG329160.12220329466260144No Hit
CGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCT327650.1216426950303845No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT323200.11999059677650015No Hit
TGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCC321080.11920352974318894No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT320440.11896592460105726No Hit
GCCAAATGCCTCGTCATCTAATTAGTGACGCGCATGAATGGATGAACGAG308420.11450340302539656No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC302360.11225357933583718No Hit
GTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTG301530.11194543516713518No Hit
AAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTATGACTCTCT296370.11002974370869847No Hit
GGGAACGTGAGCTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCT296010.10989609081624939No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG294630.10938375472852796No Hit
AAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCAC290560.10787273452778429No Hit
GGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAG289080.10732327263660478No Hit
CGTGAGACAGGTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTA278900.10354386584457269No Hit
CGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTA277100.1028756013823273No Hit
GGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAG276220.10254889431189625No Hit
GGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATT276160.10252661882982139No Hit
CGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGA272450.10114925152152682No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACT64250.038.581377
ATCCGAC64400.038.2711376
ATATTCA653350.032.4233364
TATTCAA654550.032.298855
AATATTC656050.032.210583
CGCAAAT191300.032.0733641
CGCGGTG212450.031.9532261
CCGACTG78650.031.5175258
CAAATAT676200.031.3556791
AATCCGA81650.030.0118735
GGCGATC109300.029.6043432
GTTGACG261850.029.5382466
CGACTGT83700.029.3333349
TGTTGAC265450.029.0841885
GCAAATT214950.028.7645552
TTCAAAC740350.028.4790237
CGATCTG106650.028.4769674
ATTCAAA746900.028.3369486
CAAACGA748450.028.1960969
TTGACGC278050.027.8342747
Produced by FastQC (version 0.11.4)