FastQCFastQC Report
Tue 25 Mar 2025
SH-10_S3_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-10_S3_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18200410
Sequences flagged as poor quality0
Sequence length101
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1941181.0665583907175717No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC281600.15472178923441834No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC187000.10274493816348094No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG125300.035.2329331
CGAAGCG139400.031.7370853
GCGAAGC146850.030.2558822
AAGCGGG187850.024.1056545
GAAGCGG213400.022.2616584
CGCGTGC162300.020.757721
TCGGATA29400.019.9568752
TGCGACA69200.019.8294246
GCGACAT68800.019.8071637
CGGATAG27500.019.7871533
CGATCGA26250.019.2873554
GCGTGCC173300.019.1125072
GGCGATC48850.018.984962
GCGATCT45900.018.7619043
CTCGGAT46850.017.0684991
CGATCTG48950.016.5295724
GGGCGAT58900.016.388271
GATTCAA128400.016.2145845
TGTAGTC206050.015.7991619
CCTGTAG209400.015.7949667
Produced by FastQC (version 0.11.4)