FastQCFastQC Report
Tue 25 Mar 2025
SH-10_S3_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSH-10_S3_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18200410
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGGG606110.3330199704292376No Hit
CCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGG435980.2395440542273498No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT409690.22509932468554278No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG322010.17692458576482617No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT253800.13944740805289552No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG242150.13304645334912785No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT222180.12207417305434329No Hit
CCCGGGATTCGGCGAGTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGG212710.11687099356552956No Hit
CGGGATTCGGCGAGTGCTGCTGCCGGGGGGGGCTGTAACACTCGGGGGGG200010.10989312878116482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA40850.028.7230281
TCGCTAT42200.028.4794082
CGGGATT376400.027.5378761
GAGTCTT171650.026.823774
TTCGGCG393500.026.698176
ATTCGGC398700.026.6003065
TGGAGTC177650.026.4991152
GGATTCG397650.026.3983763
TCGGCGA400500.026.2552627
GATTCGG414550.025.5489484
CCGGGAT300350.025.5112691
GGAGTCT187050.025.2414673
AGTCTTG188250.025.139875
GGGATTC427550.024.9987642
CGGCGAG440850.023.862898
GGCGAGT440900.023.7631939
CTGGAGT348500.023.145161
CGCTATG52800.022.5800913
GTCTTGG210850.022.5127936
TCTTGGA213050.022.3249247
Produced by FastQC (version 0.11.4)