FastQCFastQC Report
Tue 25 Mar 2025
Mio-9_S31_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMio-9_S31_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24041396
Sequences flagged as poor quality0
Sequence length101
%GC61

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3081541.2817641704333642No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT339610.14126051582029595No Hit
CGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTG288120.11984329029811745No Hit
CGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCT276610.11505571473470176No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA275370.1145399376974615No Hit
CGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC248820.10349648581138965No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC247570.10297654928191358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG227350.029.3821141
CGAAGCG256250.025.994593
GCGAAGC277400.025.1723692
GCGATCT60250.023.2422893
CGATCTG60650.022.5429764
AAGCGGG304800.022.3972955
GAAGCGG321200.021.7103164
GGCGATC69500.021.1699492
GCGACAT104950.020.2398877
CGATCGA123900.020.1225724
TCGGATA184200.019.468112
CGGATAG188300.018.9185943
GATCGAA141200.018.7292885
TGCGACA113150.018.4804236
CTCGGAT210550.018.2899481
TCGAAAG139550.018.2388177
ATAGCCG198350.017.8884746
GATTCAA300350.017.6729665
GCTGCGA123350.017.067314
CAGTCGA86400.017.0290664
Produced by FastQC (version 0.11.4)