FastQCFastQC Report
Tue 25 Mar 2025
Mio-7_S26_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMio-7_S26_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30562462
Sequences flagged as poor quality0
Sequence length101
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTGGGGG2063930.6753153590833094TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTGGGTT1857790.6078666044639991TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGGTT1429700.46779608265852407TruSeq Adapter, Index 8 (97% over 36bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1355710.44358664560466365No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTGGTTT1091260.3570589306581387TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTTT1066650.349006568907963TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTGGGGT983310.32173782334682327TruSeq Adapter, Index 8 (97% over 36bp)
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC939800.3075014048279226No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT875510.2864657958511327No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT872000.28531732816551236No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG851750.2786915530561641No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCGGGGGG849730.2780306115390835TruSeq Adapter, Index 8 (97% over 36bp)
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC714430.23376061784551255No Hit
CGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATA704030.2303577506288597No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGTAT682610.22334915295763805TruSeq Adapter, Index 8 (97% over 36bp)
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT637690.20865138417186418No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG626910.20512418142229508No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG613620.2007757097579377No Hit
GTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCAT557360.18236750691092884No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG536070.17540144507991537No Hit
GTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCA529440.17323211722929913No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATGGGGGGG509400.16667505386182566TruSeq Adapter, Index 8 (97% over 36bp)
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT489990.16032412571997634No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC488460.15982351160060337No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC485820.1589597068456069No Hit
CGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGG474450.15523945682124693No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA471690.1543363882137506No Hit
GTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGAC453640.1484304504002328No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCAGGGGGGGG448040.14659813728357354TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATGTGGGGG394010.1289195876955201TruSeq Adapter, Index 8 (97% over 36bp)
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT384170.1256999517905331No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC383160.12536948103199277No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTCGCGCATCTCGGGT381580.12485250697407821TruSeq Adapter, Index 8 (97% over 36bp)
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT378930.12398543023137339No Hit
TGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGC369850.12101446539221873No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC362900.11874043393493626No Hit
GAAAAAACGATCAGAGTAGTGGTATTTCACCGGCGGCCCGCAGGGCCGGC351100.11487948843911856No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA328260.10740626851331546No Hit
CCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCC307390.10057763016605142No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA305880.10008356002209506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA3216100.049.398139
AGAGCAC3361550.047.27778
AAGAGCA3421900.046.504987
CGGAAGA3582200.044.5805134
GAAGAGC3619400.044.0736736
TCGGAAG3655750.043.699393
GGAAGAG3790500.042.2284435
GATCGGA3850800.041.4651451
ATCGGAA3879350.041.2354972
CGCGCAT1674750.039.58878736-37
GCGCATC1543800.039.4162638-39
TCGCGCA1812700.039.13345336-37
CGCATCT1358350.039.0313738-39
TCTCGCG2012100.038.36902234-35
ACTCTCG2053050.037.80962832-33
CTCGCGC2019900.037.3554934-35
CTCTCGC2077300.037.2516332-33
TCGTATG84500.036.14412344-45
GTCACTC2198750.036.07214728-29
TCACTCT2218950.035.64851430-31
Produced by FastQC (version 0.11.4)