FastQCFastQC Report
Tue 25 Mar 2025
Mio-6_S25_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMio-6_S25_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30499590
Sequences flagged as poor quality0
Sequence length101
%GC79

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14913034.8895837616177795No Hit
GGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGG1037490.3401652284506119No Hit
GGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCAC856430.2808004960066676No Hit
CGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTT727480.23852123913796877No Hit
GTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCG546200.17908437457683857No Hit
CGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTCCG538790.17665483372071558No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA530730.17401217524563445No Hit
TTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAG515960.16916948719638525No Hit
GGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCG501120.1643038480189406No Hit
CGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC441930.14489702976335092No Hit
CGGCGGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTC427050.1400182756555088No Hit
GCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCTCTCCCCCC418440.13719528688746307No Hit
CTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCCTCCCCCGTCTC410340.1345395134819845No Hit
GGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCACC409960.13441492164320898No Hit
CTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGC403230.13220833460384224No Hit
GGGGAAGGTGGCTCGGGGGGCCCCGTCCGTCCGTCCGTCCGTCCTCCTCC391300.1282968066128102No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG372630.12217541284981208No Hit
GGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTC371270.12172950521629963No Hit
GGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTT365660.11989013622806077No Hit
CCCGCGTCCTCCCTCGGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCG363840.11929340689497793No Hit
CAGCACTCGCCGAATCCCGGGGCCGAGGGAGCGAGACCCGTCGCCGCGCT354770.11631959642736181No Hit
GGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG334280.10960147333128084No Hit
CTTGACTCTAGTCTGGCACGGTGAAGAGACATGAGAGGTGTAGAATAAGT326250.10696865105399778No Hit
GAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCC325840.10683422301742418No Hit
GTAGAATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGG319750.10483747486441622No Hit
CCGCGTCCTCCCTCGGGAGGGCGCGCGGGTCGGGGCGGCGGCGGCGGCGG316870.1038931998758016No Hit
TGAAGAGACATGAGAGGTGTAGAATAAGTGGGAGGCCCCCGGCGCCCCCC315360.10339811125329881No Hit
ATAAGTGGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGG311330.10207678201575823No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAGCG92850.026.8595686
GCGGAAT97750.025.5615631
AATCAGC101700.024.801425
AATCCGA24400.023.6613925
TCAGCGG107000.023.5729377
GGAATCA116450.023.4480443
ATCCGAC24600.023.0842866
GAATCAG118600.022.943174
CGGAATC124400.021.1126562
TCCGACT26650.020.7758567
CGCAAAT48950.020.5919361
GCAAATT53550.018.9998112
CGATCGA242000.018.67494
CGCGAAG698250.018.5970231
TGATTCG34600.018.464093
GATCGAA250550.018.113175
TCGAAAG241600.018.0202667
GAGCGGT366450.017.8856755
GGTCCTT89250.017.3384323
ATCGAAA265100.017.101186
Produced by FastQC (version 0.11.4)