FastQCFastQC Report
Tue 25 Mar 2025
Mio-2_S17_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMio-2_S17_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40265495
Sequences flagged as poor quality0
Sequence length101
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATG2594060.6442389445355137No Hit
CGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCA1882230.46745482701752455No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1644200.4083396963082162No Hit
CGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCCCTG1392900.34592893990251455No Hit
CTTGGACCGGCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTT1373880.34120529252154974No Hit
CTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTCCGACC1286960.319618571682777No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT1160790.2882840506493215No Hit
GACTAATCGAACCATCTAGTAGCTGGTTCCCTCCGAAGTTTCCCTCAGGA1115740.27709581119020144No Hit
TGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCC1097540.2725758121190364No Hit
GGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAAATCGGTCGTC1045440.25963669389883326No Hit
CGCAAATTACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAAT1039120.2580671118037913No Hit
GTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTC1016940.2525586733753056No Hit
CGGGTGTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTG964890.23963197273496822No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA939900.23342566631802242No Hit
CCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGA918720.22816557948685343No Hit
GGATGAACGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAG881870.21901382312573084No Hit
GACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGT877530.21793597719337612No Hit
CACGAGCGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAG873000.21681094445753119No Hit
TGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTTGGTGT869340.21590197761135183No Hit
GCAGAATTCACCAAGCGTTGGATTGTTCACCCACTAATAGGGAACGTGAG850190.21114604452273592No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA847620.21050778091763184No Hit
CCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCT822180.20418971628189345No Hit
CTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGG810990.20141066190791893No Hit
GGGGAATCCGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCG744200.18482325872313254No Hit
CACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGT738150.18332073155936615No Hit
CAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAAC734900.1825135888680867No Hit
GGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGT722930.17954082024820509No Hit
GTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAG702560.17448189821086268No Hit
CGCAGGTGCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTG688880.1710844483595694No Hit
GAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCG684380.16996686617164397No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTATTCCCCATGA669060.16616211970075123No Hit
GGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTA666610.16555365828732516No Hit
GTGGAATGCGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCT657030.16317444998503058No Hit
CCCATATCCGCAGCAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAA641480.159312582646755No Hit
CGAGATTCCCACTGTCCCTACCTACTATCCAGCGAAACCACAGCCAAGGG641010.1591958573960161No Hit
CGAGTGCCTAGTGGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATG636160.15799135214902982No Hit
GCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCATGCTAAC631930.15694082489237993No Hit
TATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAATTAGTGACGCG631750.15689612160486294No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAAC630100.15648634146929027No Hit
CCGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCC628420.1560691107857981No Hit
CGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGCAA616780.15317829819303103No Hit
CGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGG613580.1523835730816174No Hit
CGCACGTGTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAA613520.1523686719857784No Hit
GGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGACGTGC600830.1492170902158287No Hit
CGAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGT594460.14763509054092097No Hit
CGACTGTTTAATTAAAACAAAGCATCGCGAAGGCCCGCGGCGGGTGTTGA594050.1475332663860211No Hit
CTACTATCCAGCGAAACCACAGCCAAGGGAACGGGCTTGGCGGAATCAGC581720.14447109119110543No Hit
GCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTCGTCATCTAA577700.14347271776989207No Hit
TTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAAATTCAATGAAGCGCG577700.14347271776989207No Hit
CTGGGTTTAGACCGTCGTGAGACAGGTTAGTTTTACCCTACTGATGATGT573550.14244205864102752No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT571730.14199005873391102No Hit
CCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCT564850.1402813997443717No Hit
GTCAAAGTGAAGAAATTCAATGAAGCGCGGGTAAACGGCGGGAGTAACTA560420.13918120216825847No Hit
GGGAAACCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCT558430.1386869824895981No Hit
GCAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCG555090.13785748815456014No Hit
GGGTAAACGGCGGGAGTAACTATGACTCTCTTAAGGTAGCCAAATGCCTC545160.13539135679320471No Hit
CGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCA536860.13333003853547562No Hit
CAGGTCTCCAAGGTGAACAGCCTCTGGCATGTTGGAACAATGTAGGTAAG531820.13207834648499914No Hit
GTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCAGTAC516560.12828850110994536No Hit
CGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCGAAACG513120.12743417161517573No Hit
CTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGT503330.12500280947744463No Hit
CAGATCTTGGTGGTAGTAGCAAATATTCAAACGAGAACTTTGAAGGCCGA500590.12432232610079673No Hit
GAAAAATAACAATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTC486070.12071626090775736No Hit
GGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGTCCTGA483210.12000597533943144No Hit
GTTGACGCGATGTGATTTCTGCCCAGTGCTCTGAATGTCAAAGTGAAGAA478340.11879650306049883No Hit
GCAGAACTGGCGCTGCGGGATGAACCGAACGCCGGGTTAAGGCGCCCGAT473730.11765160219686856No Hit
GTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGTG472310.11729894292867876No Hit
AGCAAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCA467940.11621364644840453No Hit
CGGTAACGCAGGTGTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCG466710.1159081739837049No Hit
CGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGCCTACCCTAC461040.11450002042691888No Hit
GCCTGGGCAGGGCGAAGCCAGAGGAAACTCTGGTGGAGGTCCGTAGCGGT460940.1144751852671872No Hit
AGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCATAG447680.11118204308676698No Hit
GGGTAACCCGTTGAACCCCATTCGTGATGGGGATCGGGGATTGCAATTAT445170.1105586805775019No Hit
CAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCA439690.10919771382420607No Hit
AAATATTCAAACGAGAACTTTGAAGGCCGAAGTGGAGAAGGGTTCCATGT432630.10744435154714974No Hit
CGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAGAGCGAA427600.10619514301264645No Hit
CCGATAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGAG427060.1060610331500954No Hit
GGGAGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCT423970.1052936267143866No Hit
CGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAGGAAACTCTG421490.10467771475304102No Hit
CCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAG414160.10285729754470918No Hit
GTTAGGACCCGAAAGATGGTGAACTATGCCTGGGCAGGGCGAAGCCAGAG413300.10264371517101677No Hit
CGCCACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGGCCG405850.10079349577100691No Hit
TGTAAATCTCGCGCCGGGCCGTACCCATATCCGCAGCAGGTCTCCAAGGT404640.10049299033825364No Hit
GGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGCTACCAC403650.10024712225691006No Hit
GTTGGATTGTTCACCCACTAATAGGGAACGTGAGCTGGGTTTAGACCGTC403150.10012294645825165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCCA99500.039.513331
AGATCAG71100.038.594434
CTCAGAT70600.037.6614721
CTGCCAG144800.033.196391
TCAGACG83650.032.8606727
TCACCCG456450.028.2345033
CAGACGT99550.027.564688
TGCCAGT174950.027.448482
TCCGACT291900.026.6785347
CCTCACC482000.026.6102031
AGACGTG104000.026.476189
ATCCGAC294600.026.3858496
CTCACCC496150.025.9180722
CCGACTG311450.025.0798518
ACCTCAC269950.024.9219766
AATCCGA311950.024.7363265
AACCTCA279500.023.9011675
GCCAGTA210450.022.9981523
CGGGAAA398750.022.211141
CCAGTAG220400.022.0028274
Produced by FastQC (version 0.11.4)