FastQCFastQC Report
Tue 25 Mar 2025
Mio-2_S17_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMio-2_S17_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40265495
Sequences flagged as poor quality0
Sequence length101
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA2766180.6869852214656743No Hit
GTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAAC2540140.6308478264081939No Hit
CGCGTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATA1968280.4888254819666317No Hit
CGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGG1592480.39549495169499344No Hit
GTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGAGG1589560.39476976503082845No Hit
GCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCG1520260.37755899933677706No Hit
CGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGC1390020.3452136873022423No Hit
CTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCG1368780.3399386993752343No Hit
CGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCA1202240.29857822435810116No Hit
CGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGC1114320.27674315192201165No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA1104080.27420003156548806No Hit
CCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAG1074930.26696058250370447No Hit
CTTCACCGTGCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGC1046750.25996203449131816No Hit
GAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACTC1025730.2547416839157199No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA1015300.2521513767557061No Hit
GTCGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTC1011280.2511530033344927No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA981840.2438415323094873No Hit
CCGGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGG963690.23933395081818812No Hit
CGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAG960440.23852680812690868No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCT947050.23520138023883724No Hit
CTCGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACAT942390.23404406179534115No Hit
GTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCT933330.23179399632365127No Hit
CGCCCCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACG927790.23041812847451648No Hit
CCCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCA919640.22839406295638484No Hit
CGGGGGTGCGTCGGGTCTGCGAGAGCGCCAGCTATCCTGAGGGAAACTTC875580.2174516915786084No Hit
CCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCA864880.21479432948731914No Hit
CCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAA857710.21301364853455793No Hit
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT855210.21239276954126604No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA842780.20930575918661873No Hit
CAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATTCCT836780.20781564960271814No Hit
CTACGAATGGTTTAGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACGG834670.20729162773237977No Hit
GAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGA813200.20195951893798897No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACC813080.20192971674631097No Hit
CGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCT810910.20139079378013358No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGAT794400.1972905089084339No Hit
CCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCTCCAGCGC788580.19584510261205032No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC777470.19308591636586114No Hit
TGCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACT774900.19244765276075707No Hit
GTCACTAATTAGATGACGAGGCATTTGGCTACCTTAAGAGAGTCATAGTT771490.19160077381390692No Hit
GGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGA750950.18649963200502068No Hit
TGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGACACT704490.174961216793684No Hit
GCGAGAGCGCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTA700180.17389082140924877No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC692180.1719040086307147No Hit
GGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATG689830.17132038237702032No Hit
CAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGG684330.16995444859177813No Hit
GCCGACTTCCCTTACCTACATTGTTCCAACATGCCAGAGGCTGTTCACCT671240.16670352618290174No Hit
TGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGGC648580.161075878987704No Hit
GTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCA643140.1597248462983008No Hit
ATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCC640460.1590592640174919No Hit
CCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGCC630290.15653352827278044No Hit
CGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCGA625120.1552495505146528No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC619610.15388113321343747No Hit
GCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCAC617760.15342168275840146No Hit
CGCTCGCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCAC606470.15061779322469523No Hit
CTTAAGAGAGTCATAGTTACTCCCGCCGTTTACCCGCGCTTCATTGAATT605900.15047623281422468No Hit
GTACAAAGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACT604090.15002671642308135No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC594870.14773691469582081No Hit
GCCAGACTAGAGTCAAGCTCAACAGGGTCTTCTTTCCCCGCTGATTCCGC591820.14697944232400473No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC588850.14624183807997393No Hit
CTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCTATTCCATTATTC587920.14601087109446934No Hit
AGCAACTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCA587130.1458146733325891No Hit
TTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGCC587000.14578238762493792No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA581200.1443419483605007No Hit
GTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCG577170.1433410914233142No Hit
GCCCATCTCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGA574830.14275994868559297No Hit
CTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATCGGTC571220.14186339941927945No Hit
GGGCTTCTTACCCATTTAAAGTTTGAGAATAGGTTGAGATCGTTTCGGCC567600.1409643666369928No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT559740.13901232308208306No Hit
CCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAA557910.13855783965899338No Hit
CCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGT553810.13753959810999467No Hit
CCGAAGTTACGGATCCGGCTTGCCGACTTCCCTTACCTACATTGTTCCAA551730.1370230267875758No Hit
CCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCG549300.13641953240609608No Hit
GCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGG547940.1360817742337453No Hit
CCCTGTGGTAACTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGA547610.13599981820663076No Hit
CTTCGCCCCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACC541270.1344252690796425No Hit
TATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTG537800.13356348903695336No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAACGGCCATGCAC536650.13327788470003907No Hit
GGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTC531360.13196410475023343No Hit
ACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTC528340.13121408292633682No Hit
GGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCC525520.13051373142190353No Hit
CTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTG520880.12936138001035377No Hit
GTGGGAATCTCGTTCATCCATTCATGCGCGTCACTAATTAGATGACGAGG517840.12860639115451084No Hit
CTGCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTC513980.12764775398886813No Hit
CCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGGAGA513320.12748384193463907No Hit
CCGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGA511230.12696478709624703No Hit
CCGGATTTTCAAGGGCCAGCGAGAGCTCACCGGACGCCGCCGGAACCGCG506540.12580001810483143No Hit
CGCCACTCCGGATTCGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGA506240.12572551262563642No Hit
GCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGC501970.12466505130509385No Hit
CGCCGGTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCT498600.12382810642213639No Hit
GTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCAT496670.12334878783931502No Hit
CTGAACGCCACTTGTCCCTCTAAGAAGTTGGGGGACGCCGACCGCTCGGG493880.12265588688280125No Hit
CCATTGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTT489690.12161529369004404No Hit
GAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGCCC480170.1192509864835885No Hit
CTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCCCAAGCAACCCGA479410.11906223926962775No Hit
GGATAGTAGGTAGGGACAGTGGGAATCTCGTTCATCCATTCATGCGCGTC479390.11905727223768142No Hit
CGGAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTT477550.11860030529861859No Hit
GTCGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAA473770.11766153626076123No Hit
CGGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCC468760.11641729475820425No Hit
CGGCATCGTTTATGGTCGGAACTACGACGGTATCTGATCGTCTTCGAACC467730.11616149261296799No Hit
CCCCGCCTCACCGGGTCAGTGAAAAAACGATCAGAGTAGTGGTATTTCAC466520.11586098718021473No Hit
CTTTTACTTCCTCTAGATAGTCAAGTTCGACCGTCTTCTCAGCGCTCCGC458470.1138617568218148No Hit
CCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCTCCACC457060.11351158106959819No Hit
CGCCCGTCGGCATGTATTAGCTCTAGAATTACCACAGTTATCCAAGTAGG456770.1134395591063763No Hit
CCCGCTTTCACGGTCTGTATTCGTACTGAAAATCAAGATCAAGCGAGCTT456020.11325329540838876No Hit
CGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGATTCGGCAGGTGAG455910.1132259767326839No Hit
CTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCATCGCCCGTCCCTTCGG454780.11294533942771597No Hit
TCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCC446850.11097591126099406No Hit
CGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTC445540.11065057066850911No Hit
CTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTAC444480.11038731797535335No Hit
CCCAGCCCGACCGACCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTAC442530.10990303236058566No Hit
CCCAGCCCTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCC441780.1097167686625981No Hit
CGGGTATGGGCCCGACGCTCCAGCGCCATCCATTTTCAGGGCTAGTTGAT440290.10934672478259612No Hit
CCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTA437820.10873329633722371No Hit
CTTTTCTGACACCTCCTGCTTAAAACCCAAAAGGTCAGAAGGATCGTGAG434120.1078143954271517No Hit
CTCCCGTCCACTCTCGACTGCCGGCGACGGCCGGGTATGGGCCCGACGCT430740.10697496702822107No Hit
CTAGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCT429770.10673406597882382No Hit
CGGGGGCCTCCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAG428890.10651551657318505No Hit
CTGACTTAGAGGCGTTCAGTCATAATCCCACAGATGGTAGCTTCGCCCCA428630.1064509451578827No Hit
TGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGG424960.10553949479573019No Hit
CTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACCT419640.10421826429800503No Hit
CCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCAC418100.10383580283813722No Hit
GCCAGCTATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTC417870.10377868197075435No Hit
GCCAGGTTCCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGC416880.10353281388941077No Hit
CCACCGTCCTGCTGTCTATATCAACCAACACCTTTTCTGGGGTCTGATGA415700.103239759004577No Hit
CTTGGCTGTGGTTTCGCTGGATAGTAGGTAGGGACAGTGGGAATCTCGTT411460.10218674823195395No Hit
GGGGATCTGAACCCGACTCCCTTTCGATCGGCCGAGGGCAACGGAGGCCA410010.10182663841584462No Hit
CTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTCCT406660.1009946605648335No Hit
CCCACTTATTCTACACCTCTCATGTCTCTTCACCGTGCCAGACTAGAGTC406060.10084564960644343No Hit
CTCGTTCGTTATCGGAATTAACCAGACAAATCGCTCCACCAACTAAGAAC405510.10070905622791922No Hit
TGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGGTTCATCCCGCAGC405130.10061468262093885No Hit
GCGAGATTTACACCCTCTCCCCCGGATTTTCAAGGGCCAGCGAGAGCTCA405040.10059233097718034No Hit
CCAAGACCTCTAATCATTCGCTTTACCGGATAAAACTGCGTGGCGGGGGT403980.10032907828402457No Hit
CAGACTTGCCCTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCAT403710.10026202335274904No Hit
CTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTCT403130.10011797942630532No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCGAG46150.063.093885
CTTGTGT48400.059.8714261
TGTGTCG51100.056.889023
TTGTGTC63950.045.3126032
GGCACAT14250.043.3374751
GTCGAGG67300.043.12456
CGCAGAC12750.042.8472371
TGCACAT14600.042.2985651
GTGTCGA72300.040.273824
TGCCCCT23300.038.125961
CCGGACT22000.037.5717741
TCGTATC4050.036.361052
CGGCTAC18400.035.8865851
CGCGTAA1331500.035.727411
GCGTAAC1357850.035.1805952
CGTAACT1360900.035.0607873
CTCCCGT154400.033.9054451
GCAGACC15250.033.3306432
TCCCGTC156500.033.2679442
AACTAGT1439700.033.2209666
Produced by FastQC (version 0.11.4)