FastQCFastQC Report
Tue 25 Mar 2025
Mio-16_S30_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMio-16_S30_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22604965
Sequences flagged as poor quality0
Sequence length101
%GC68

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3568351.5785691329316371No Hit
CGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC470480.2081312667371969No Hit
CGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTT332260.14698540785177064No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA321970.14243331055810085No Hit
GGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTTCCAC266170.11774846809097028No Hit
GGGAGGCCCCCGGCGCCCCCCCGGTGTCCCCGCGAGGGGCCCGGGGCGGG242840.10742772660784922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG463550.030.2477651
CGAAGCG505350.027.7270953
GCGAAGC551450.026.1471612
GAAGCGG551700.025.8608384
AAGCGGG588950.024.3858835
TCGGATA181250.023.4975572
CGGATAG186800.022.4447673
CGATCGA163250.022.233124
CTCGGAT206850.021.6875781
ATAGCCG196000.021.3670926
TCGAAAG169200.021.0317657
GATCGAA176650.020.7609065
TAGCCGG210250.019.9864317
GATAGCC214050.019.941125
GAGCGGT201850.019.8100785
ATCGAAA188000.019.708896
GCGATGT55500.019.4401722
CGAAAGG188150.019.0644048
AGCCGGT223900.018.6622878
GATTCAA522950.018.577495
Produced by FastQC (version 0.11.4)