FastQCFastQC Report
Tue 25 Mar 2025
Mio-15_S29_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMio-15_S29_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20200265
Sequences flagged as poor quality0
Sequence length101
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2872351.4219367914232808No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA245070.12132019060145995No Hit
CGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC229910.11381533856115254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG208550.031.2656381
CGAAGCG234450.027.8318653
GCGAAGC242800.027.5178382
AAGCGGG278450.023.9777325
GAAGCGG297100.023.0140724
TCGGATA91900.022.7580492
CGGATAG92650.022.1652473
AGCGGTC108800.021.26716
CGATCGA65700.020.8142494
CTCGGAT110450.020.4352971
GCGATCT38200.020.3146253
GATTCAA245850.020.0551435
ATAGCCG103450.019.9427156
GAGCGGT123300.018.9963595
GGATTCA265450.018.8417194
ATTCAAC260500.018.6184886
GATCGAA81450.018.2417645
GAGGATT277450.018.0609052
TCGGTTG74700.018.0531313
AGGATTC273750.017.8901713
Produced by FastQC (version 0.11.4)