FastQCFastQC Report
Tue 25 Mar 2025
Mio-14_S32_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMio-14_S32_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29191709
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1448060.49605180703877255No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC457050.15656842838492258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGGTG170250.019.8713421
CGCGTGC211150.019.3387321
GCGTGCC241200.016.733242
GGCGCGT171750.015.5653927
CGGGAAT71100.015.306171
CGGGGAT96750.015.2095981
TGGCGCG190450.014.6830016
CGTGCCT285950.014.28002553
GCGCGTG204000.013.4294028
CGACTAA44050.013.42677328-29
TAGTCCC301800.013.3262519
GTAGTCC314900.012.9972588
GTGGCGC223950.012.8246615
CCTGTAG333500.012.6412547
CGGTGGC250050.012.4510893
TGTAGTC339300.012.4251647
GGGAAAT254850.012.2165781
CTTGAGC308600.012.1279921
CGCCAAT54200.012.04725551
GGGGAAT178150.011.9252861
Produced by FastQC (version 0.11.4)