FastQCFastQC Report
Tue 25 Mar 2025
Mio-14_S32_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMio-14_S32_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29191709
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG589000.20176961890103795No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA49150.024.258871
TCGCTAT51050.023.262582
AGCTATC189100.022.53114738-39
GCTATCT176750.022.17057438-39
GAAGCTA227650.021.0108836-37
CTGGAGT532050.020.999321
GGAGTGC349050.020.7659723
GAGTGCA368150.020.1085744
AAGCTAT243600.019.56692136-37
TATCTCG70900.018.95924840-41
GTGCAGT380900.018.8865266
GAGCACA628200.018.5464049
AGAGCAC637800.018.0511998
AGTGCAG438250.016.9571155
CGGAAGA682350.016.9562744
TGGAGTG451800.016.9275192
TCGGAAG688700.016.6767583
CGTCTGA355250.016.5927616-17
AGTCACC365650.016.27020628-29
ATCGGAA711150.016.064512
Produced by FastQC (version 0.11.4)