FastQCFastQC Report
Tue 25 Mar 2025
Mio-11_S2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMio-11_S2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284
Sequences flagged as poor quality0
Sequence length101
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGGT682.9772329246935203TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGTT662.8896672504378285TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTTT180.7880910683012259TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTGGGTT160.7005253940455342TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTGGGGT140.6129597197898424TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTGGTTT100.43782837127845886TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGCGGGT90.39404553415061294TruSeq Adapter, Index 6 (97% over 36bp)
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT90.39404553415061294No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTGT70.3064798598949212TruSeq Adapter, Index 6 (97% over 36bp)
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC60.2626970227670753No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGCGGTT60.2626970227670753TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTGGTGT60.2626970227670753TruSeq Adapter, Index 6 (97% over 36bp)
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG60.2626970227670753No Hit
GCCACACACGGCCATGCAGTACTCTTCTGTGTCAAAGTTGTTCCGGTTGC50.21891418563922943No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT50.21891418563922943No Hit
GGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCAC50.21891418563922943No Hit
GCTGATTCCTAGGAATGTCTTACATCTCAAGCTGAATGTCAGGTTCCTCC50.21891418563922943No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG50.21891418563922943No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT40.17513134851138354No Hit
GGCATCGTCGCCCGCGAAGCCGGCCTTGCACATGCCGGAGCCGTTGTCGA40.17513134851138354No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCTCTCCGGAGAATCTCGGGT40.17513134851138354TruSeq Adapter, Index 3 (97% over 34bp)
GGCTTGTGGTCTTGGAGAGCCCTTGCCCTTGAGCTTCTCTTCCTCTTCCT40.17513134851138354No Hit
CCCGTCTCTACTAAAAATACAAAATTAGCTGGGTGTAGTGGCCTGTAATC40.17513134851138354No Hit
CTTCCTCTTGTACATGGCCTTTCTGGAATACATGGCAGATCGGGAATACC40.17513134851138354No Hit
CCAATTTCTGCAGAGTATCAAATTGTGCTCTGGTTGTGATATAGTATCCA40.17513134851138354No Hit
CTGTACCCGATCTTGAACTTCCTCATGAACACCTTGTTCTCTCGGTGCTT40.17513134851138354No Hit
CGGGTCTCTCGCGCGAGCGATTTAGTCTGAGGCGAAGCTTCGGAGCGGCC40.17513134851138354No Hit
GCCGGATCTCCCGCAGGGCTACGGTGCCGGGCCGGTAGCGGTGCGGCTTC40.17513134851138354No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA40.17513134851138354No Hit
GGGGCAGCCTGCCCTTGTTCTTTTTGTCGTGTGTCCTTCACATGCAGTAA40.17513134851138354No Hit
GTCATCCTGCAAAGTGCGTATCAAAAAATACGAAGTTAGGGTGACAAAGT40.17513134851138354No Hit
GCGCTTTTCTAGTGTACATTACGACTTACAACATAACTGTTAACTAATTT40.17513134851138354No Hit
CACGCCTGTAGTCCCAGCACTTTGGGAGGCTGAGGCAGGCAGATCACGAG40.17513134851138354No Hit
GTCCAAGTCCAGGCCCTGACATTCATAGCGCCCACTGTGTTCCTTCCGGG40.17513134851138354No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC40.17513134851138354No Hit
GTCCAACTTTAGCTCTGCTTCAGGGCTCTGAGGTGAGGAGCAGGGGCCTT40.17513134851138354No Hit
GTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGAC40.17513134851138354No Hit
TGGCTCTAAGGGGACGTCATGGACTTGAGCTTCTCACGCAGGTCCGTGTA40.17513134851138354No Hit
AAAAGCTTGGACACAGTGGCTCACACCTGTAATCCCAGCACTTTGGGAGG30.13134851138353765No Hit
TGAAGACTTTGCTTTTCTTGAACAATCTCATTTTCAAGAATCTCAACCTT30.13134851138353765No Hit
CCAGCTCCGGGACCTCGGGGACCACAGCGTGGCCGCCGCCTAAGGGGCCT30.13134851138353765No Hit
TGACATACAGGTAGGCTCTATAGCAACAGGCCTGGAGGTGCTGCAGTAGT30.13134851138353765No Hit
CTGGGGTCTTGGTAGGACGCTGATCTCAGAAGTCCCCAAAGTTCACTGTG30.13134851138353765No Hit
GCCAGAGTTTGAATCTAGCTCCTCCACTTGCTGGCTATGTGACTTAGGCA30.13134851138353765No Hit
GGTAGGGACAGATCCTGTTTCTCTTTGTTTCAGGGCTCTGAAAGCCGGAG30.13134851138353765No Hit
ATTCACGGTAGCTTTCTTCGCTTCTTTAGTGATGGCATCTGCAGTCTCTT30.13134851138353765No Hit
CCTGACTTCTCCTCCTCCAGCTCCTCTGTCTCTGGACACATGGCCCTCCC30.13134851138353765No Hit
CTTGTGTCCACTATATTCCCGGCACCCGGAACAGGAGGGCAGGGGTCTTG30.13134851138353765No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC30.13134851138353765No Hit
GAGAAAATGGGTCAGATGGGGGAACAGGAGGTTCACTGCTGTTATTGTAA30.13134851138353765No Hit
ATATAAATCCCTTTACACCTCAGTACGAAACTCCCCCATTATATTAGAAA30.13134851138353765No Hit
TGTCACAGTCACATATAGTGCGTCCTAGAAAAGAGGAGGGGCAAGATGGG30.13134851138353765No Hit
CGGACATGGTGGCGGGCGCCTGTAGTCCCAGCTACTCAGGAGGCTGAGGC30.13134851138353765No Hit
GGCACTTTCAGAGACCAAGGTGGGAGGCTAGCTTTGAGGCCAAGAGTTTG30.13134851138353765No Hit
CCTGGGATACTCAACAGGGAGTATTTGCACATATTTCAAAATCTAAAAGA30.13134851138353765No Hit
GCACCAGCCACCCTGCCTGGAGGACTCTCTCAGCCACTGCTACTCCCACG30.13134851138353765No Hit
CTGGGTCATCCATTCCTGCATCTCTGCCAAGTCTTTCTTCAGGTTCGCAG30.13134851138353765No Hit
AGAAAAACCAGGCTTTTTTAAAAAACACGTTTGTTATTACCAAAAAGAGA30.13134851138353765No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA30.13134851138353765No Hit
CAAAACTCACGGGCCTCTTTGGTCTGCATGATGGCCCCATGCGTTTCATA30.13134851138353765No Hit
GGACGGGGAGCGGGGGAGAGAGAGAGAGAGAGAGGGCGCGGGGTGGGGAG30.13134851138353765No Hit
GCAGGAGAGAGGACTTGGAGTCGATGGACAGGGAACTCGAATTACACTGA30.13134851138353765No Hit
GCAAATCTTCTTTACTTCATCTCCTGTCAGGGCATACTTAAGTCTGTTCC30.13134851138353765No Hit
GTGGGTTACAAATCAGATCTGGTGACAACACTGAGGGGGCCAGGTCAGGG30.13134851138353765No Hit
CTCCGTCTCTAATAAAAATACAAAAATTAGTCGGGCATGGTGGCGTACGC30.13134851138353765No Hit
GTTATAATTAAGGAGATTTGTAGGGAGATTAGTATAGAGAGGTAGAGTTT30.13134851138353765No Hit
GCCCTCTGCGCTGGGTCAAATACCAACCAGTCAATTACATCAATTTTTAA30.13134851138353765No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT30.13134851138353765No Hit
TGCAAGGCAGCCAATAAGAGGGTCATGGATGTGATCAACTCAGCCAGGAC30.13134851138353765No Hit
CTCTAAATTAGGTTCTCTGGGTTCAATTTTCTCCTCTAATTTTTCCCTTT30.13134851138353765No Hit
ATTGAGGAGGCCAACCTGCAGTGGCCCAGAAGCAAACAGAGGTCTCCCAT30.13134851138353765No Hit
AAGAGATAAAGAAAATCCAAACCAGTACAACTATATTGCATAGAATGTTG30.13134851138353765No Hit
TTTGTTTCCTGTAGGATCCTCATCTACTTCATCATTGGTGTTAGGTGGGA30.13134851138353765No Hit
TAGAGTTTTTATTATTCAAAATGAGTTTCATTTCAATTCAGGTACTTGAA30.13134851138353765No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTGGGTTT30.13134851138353765TruSeq Adapter, Index 6 (97% over 35bp)
ATTTTCGTATGTTCAAAAATGGATCTTTTTGGAGGCCACCACCATTAAAT30.13134851138353765No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC30.13134851138353765No Hit
CTTGGGTGCTGCCCACCACTAGGACCTTGTGTACCCGGATAAGTCCCACA30.13134851138353765No Hit
CTTATCAACAGGCTGCTGGGCCTTAATACTGACAGTGAGTGTGGCGGGTC30.13134851138353765No Hit
ATACTGTTAAACATCCTGTAATGCACAGGGCAGTCAGCCTCCGTGCACCT30.13134851138353765No Hit
TGATGGTCTTCTGCATTTCCAGAACAAACTCTCTGAAGTCATTTGTCCCA30.13134851138353765No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA30.13134851138353765No Hit
AGATAAGGGAATGGATCCACAGTCCATCAGGGAAAACCACACATCTACAG30.13134851138353765No Hit
CTCACACCTGTAATCCCAGCACTTCAGGAGGCTGAGGCAGGTGGATCATG30.13134851138353765No Hit
CCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGGAG30.13134851138353765No Hit
CCCACCTCAGCCTCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGA30.13134851138353765No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA30.13134851138353765No Hit
GTCCACGGCCCCCTCGACCTCTTCCAAGACCACCACGACCTCGAATAGGT30.13134851138353765No Hit
TGGAAAGCATCCCTAGCGAAGGGGACTTGGGCTCATGGCTCATGCCTCTG30.13134851138353765No Hit
CTTCACCAAGGCTGATGTCTGGGGCACTGACACCCCCTGAAACATCCGCA30.13134851138353765No Hit
TCTGGGTCCACAGGAGCTTTGCCCTTTAACAGCAAGGCCTTCACAGATTC30.13134851138353765No Hit
GTCATACTATACCAAGCCTCTACAGACATACATAGGGACTTCAAGAGATT30.13134851138353765No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGAT30.13134851138353765TruSeq Adapter, Index 6 (97% over 36bp)
GGCCCAAATGCCCTAAGGCGGTATACCCCATACATCATCACATGCATCTG30.13134851138353765No Hit
CTCCATGCTACGGACCAACACAGTATTGAGTCAACTGTGACCTTAAGATC30.13134851138353765No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGGT30.13134851138353765TruSeq Adapter, Index 6 (97% over 36bp)
GCGAGGACTGCTTGAAGGACTTCTGGCACACGCCGCAGGGGAAGCGCCGC30.13134851138353765No Hit
GTTTTTCTCTTTTCCAAGATGGCCGATGTTATGGTTTTCTACGAAGTCAG30.13134851138353765No Hit
CTGAAGATACCAACAATTTTTCACTTCCTTGTGTTTGGCTCAAAGTTGCC30.13134851138353765No Hit
ATACAACATAGCACAACTCCATTTGTTGTTTTTCGTTGTGCTTTGTAGAG30.13134851138353765No Hit
CAAGAGTATGAGGACTTGCTAGGTCCTGCATTTCCATAGACCTGGAATTC30.13134851138353765No Hit
ATTCGTTCTCTGTTTCAGAAACCTTAACTGTGTGCCTTTGGGGGAAAGCG30.13134851138353765No Hit
GAAAAAACACATCACCTCTCCCCTACAAGCCCCCTTGCCCCAGGCAGCAT30.13134851138353765No Hit
CTGCAATACCAGGTCGATGCGTGGAGTGGACGGAGCAAGCTCCTATTCCA30.13134851138353765No Hit
CTTGGGGCCATCGCTGCCTGGGCTGCCCTTGGTGCCGGGGTCTCCCCTGC30.13134851138353765No Hit
TTTTTATTTTTTGAGATGGAGTTTTGTTCTTGTTGCCCAGGCTGGAGTGC30.13134851138353765No Hit
GGATAATATTCATTTAGCCTTCTGAGCTTTCTGGGCAGACTTGGTGACCT30.13134851138353765No Hit
AGATTTTCTATCCAGTCTAGGAAAAGTAAAGCACCTTTGGATTAAAAAGA30.13134851138353765No Hit
ATAGCTTAACAGTCAAACACACCAAGTTCTATAAAATAAAAATTAAAACA30.13134851138353765No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGAATC150.00967454947.538-39
GGAGAAT150.00967454947.536-37
CCGGAGA150.00967454947.534-35
CGGAGAA150.00967454947.536-37
AGAATCT150.00967454947.538-39
AGAGATC150.00967454947.576-77
Produced by FastQC (version 0.11.4)