FastQCFastQC Report
Tue 25 Mar 2025
Mio-10_S28_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMio-10_S28_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32310631
Sequences flagged as poor quality0
Sequence length101
%GC66

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5277201.6332704861133787No Hit
CGCGAAGCGGGGCTGGGCGCGCGCCGCGGCTGGACGAGGCGCCGCCGCCC617200.1910207200843586No Hit
CTTTTCTTTGTGAAGGGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGA525370.1625997338151644No Hit
CGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTT327370.10131959354182837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAG748700.025.3186511
TCGGATA177200.023.5527882
GCGAAGC822100.023.495562
CGAAGCG815050.023.3562583
CGGATAG181650.022.8195023
TCGGGCT395750.022.4310382
CTCGGAT201400.022.038441
GAAGCGG901500.021.6571334
ATAGCCG192050.021.5098516
AAGCGGG900150.021.4688285
GATAGCC216150.019.3742775
AGCGGTC245650.019.3206466
TAGCCGG217050.019.1849357
CGATCGA145900.018.746064
GAGCGGT264400.018.666395
AGCCGGT223650.018.534158
GGATAGC235050.017.8365544
GATTCAA547100.017.6701055
CGGGGAG744850.017.2988131
GGATTCA567750.017.2941194
Produced by FastQC (version 0.11.4)