FastQCFastQC Report
Fri 17 Dec 2021
L-DM9-Plus_S5_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM9-Plus_S5_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85191324
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT7652900.8983191762579015No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2605510.3058421770742758No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2031100.23841629694591904No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1487820.17464454478956096No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400120.16435006926292164No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1319450.1548807951382467No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1118410.13128214793328016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT942500.11063333162893442No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT862290.1012180536130651No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG1729200.052.504921
CGTGTGC391950.049.7030141
GCAGTGG1921900.047.4316142
CAGTGGT2146850.042.545943
TGTGCTC483700.040.0020753
GTGTGCT495050.039.200532
AGTGGTA2899400.031.6144814
GTGCTCT688100.028.1784364
GTGGTAT3483350.026.3213445
TGGTATC3573050.025.6724156
AAGCAGT445700.025.4504091
CGACGCT69250.024.7371461
GGTATCA4106550.022.3806537
GTATCAA4448250.020.7311028
TATCAAC4626250.019.9665479
TCTTCCG1526300.019.6216681
CTTCCGA1621000.018.3875182
TGCTCTT1034600.018.008855
GCTCTTC1032050.017.9503236
TTCCGAT1733800.017.2375453
Produced by FastQC (version 0.11.4)