FastQCFastQC Report
Fri 17 Dec 2021
L-DM7-Next_S14_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM7-Next_S14_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37128815
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAGAATGAGTTATCTTCAGTCTCACCGTCCGCGTAAACGCGA1387740.3737636119008915No Hit
ATATTATATGTCCCAGTGAGATTGCACCCCGTGTGCTAGAGGCACTTGCC1261880.33986541181020724No Hit
ATATAATATCGTCCCCGTAGATGCCTATGGTTCCGGCGTTACCAAAATGG1086660.2926729549542586No Hit
GGTTTGACCTGTGCGAGCTTTTAGTACCCTTGATAGGGAGAACGAGACCT873090.23515159317635104No Hit
TTTTTAATGTCTTTAGCGAGACGCTACCATGGCTATCGCTGTAGGTAGCC774060.20847958654215062No Hit
GTCTTTAGCGAGACGCTACCATGGCTATCGCTGTAGGTAGCCGGAATTCC759090.20444767763258806No Hit
CTTTCGGGGTCCTGCTCAACTTCCTGTCGAGCTAATGCCATTTTTAATGT734960.1979486821758249No Hit
ACCATGGCTATCGCTGTAGGTAGCCGGAATTCCATTCCTAGGAGGTTTGA703610.18950510540129006No Hit
GTTTCGTTTTGGCCCCAGTCGAGTTAAAACGACCGGGAGTCCAGTTCGAA686590.18492106467712477No Hit
CTACCATGGCTATCGCTGTAGGTAGCCGGAATTCCATTCCTAGGAGGTTT679190.18292800349270505No Hit
CCTAGGAGGTTTGACCTGTGCGAGCTTTTAGTACCCTTGATAGGGAGAAC620550.16713434026914137No Hit
GGCTATCGCTGTAGGTAGCCGGAATTCCATTCCTAGGAGGTTTGACCTGT602640.1623105935376607No Hit
CTTTTAGTACCCTTGATAGGGAGAACGAGACCTTCGTCCCCTCCGTTCGC600560.16175038174528328No Hit
ATGTGACACTTAACGCCCCCCGTGAATACGGAGAGGGGTAGTGCCACTGT535210.14414949682611738No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT528480.14233688847866544No Hit
GCTCAACTTCCTGTCGAGCTAATGCCATTTTTAATGTCTTTAGCGAGACG517450.1393661499835101No Hit
GGGTAGTGCCACTGTTTCGTTTTGGCCCCAGTCGAGTTAAAACGACCGGG502420.13531808111839821No Hit
ACTTCCTGTCGAGCTAATGCCATTTTTAATGTCTTTAGCGAGACGCTACC492850.13274056820827704No Hit
CTTAACGCCCCCCGTGAATACGGAGAGGGGTAGTGCCACTGTTTCGTTTT465620.12540664171479754No Hit
GAGAGGGGTAGTGCCACTGTTTCGTTTTGGCCCCAGTCGAGTTAAAACGA464440.1250888292556603No Hit
CCTTGATCTATCGATGTGACACTTAACGCCCCCCGTGAATACGGAGAGGG451100.12149593247185507No Hit
AGTTAAAACGACCGGGAGTCCAGTTCGAACGATATTTTAAAGAGAATGAG441360.11887263302101077No Hit
CTATTTATCTGACCGCACCGATCATTCGCCTCCCGTAGCTTAGCGATAGC438430.11808348852501757No Hit
GTGCGAGCTTTTAGTACCCTTGATAGGGAGAACGAGACCTTCGTCCCCTC427130.11504003022989018No Hit
GATCTATCGATGTGACACTTAACGCCCCCCGTGAATACGGAGAGGGGTAG413770.11144174679423514No Hit
GTTATCTTCAGTCTCACCGTCCGCGTAAACGCGAACGGAGGGGACGAAGG412600.11112662766102285No Hit
GATAAATAGAGAAGGTTTCTTACATGACAAATCCTTGTCATGGGATCCGG407880.10985537782447408No Hit
GTCTCACCGTCCGCGTAAACGCGAACGGAGGGGACGAAGGTCTCGTTCTC393630.1060173883815037No Hit
GAGTTATCTTCAGTCTCACCGTCCGCGTAAACGCGAACGGAGGGGACGAA386280.10403779382670846No Hit
CCTTTCGGGGTCCTGCTCAACTTCCTGTCGAGCTAATGCCATTTTTAATG386180.10401086056745955No Hit
GGTCAAACCTCCTAGGAATGGAATTCCGGCTACCTACAGCGATAGCCATG384530.10356646178985243No Hit
GTCCTGCTCAACTTCCTGTCGAGCTAATGCCATTTTTAATGTCTTTAGCG377860.10177001339794982No Hit
GTATCAACGCAGAGTACGGGCGACCCCACGATGACCCACTTCGCTTGTAG371860.1001540178430149No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA781150.062.4466821
GTATCAA2325650.046.6960261
TATGTCC281750.036.5383647
ATGTCCC292850.035.1169748
ATATGTC295650.034.882186
TATTATA319700.034.6214142
TTATATG308000.034.3855324
TATATGT313750.033.5854075
ATTATAT323550.033.498143
ATATTAT336400.032.843871
TATCAAC3401550.031.9218372
ACTATTG255800.030.96577366
ATCAACG3569600.030.4383643
TGGGTCG270850.030.42889254
GACTATT263100.030.34673165
GTGAGAT345350.030.3397816
CCCGTGT332250.030.10916328
TGTCCCA342350.030.030049
CCGTGTG336600.029.98030529
CAACGCA3644250.029.8639975
Produced by FastQC (version 0.11.4)