FastQCFastQC Report
Fri 17 Dec 2021
L-DM7-Next_S14_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM7-Next_S14_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37128815
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTTAAAGAGAATGAGTTATCTTCAGTCTCACCGTCCGCGTAAACGCGA1529670.41198998675287646No Hit
ATATTATATGTCCCAGTGAGATTGCACCCCGTGTGCTAGAGGCACTTGCC1266170.3410208486319857No Hit
ATATAATATCGTCCCCGTAGATGCCTATGGTTCCGGCGTTACCAAAATGG1115210.30036240046982376No Hit
GTATCAACGCAGAGTACGGGCGACCCCACGATGACCCACTTCGCTTGTAG1089930.29355367253169806No Hit
TATCAACGCAGAGTACGGGCGACCCCACGATGACCCACTTCGCTTGTAGG759770.20463082379548067No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT709460.1910807010673516No Hit
CTACCATGGCTATCGCTGTAGGTAGCCGGAATTCCATTCCTAGGAGGTTT707620.19058512909717157No Hit
ACCATGGCTATCGCTGTAGGTAGCCGGAATTCCATTCCTAGGAGGTTTGA692540.1865235936024352No Hit
GTTTCGTTTTGGCCCCAGTCGAGTTAAAACGACCGGGAGTCCAGTTCGAA691920.18635660739509194No Hit
GGTTTGACCTGTGCGAGCTTTTAGTACCCTTGATAGGGAGAACGAGACCT686500.18489682474380076No Hit
GAGTACGGGCGACCCCACGATGACCCACTTCGCTTGTAGGCACCTTGATC669620.1803504905825839No Hit
GTCTTTAGCGAGACGCTACCATGGCTATCGCTGTAGGTAGCCGGAATTCC653780.17608426231755578No Hit
CTTTCGGGGTCCTGCTCAACTTCCTGTCGAGCTAATGCCATTTTTAATGT650490.17519815808826647No Hit
CCTAGGAGGTTTGACCTGTGCGAGCTTTTAGTACCCTTGATAGGGAGAAC602090.16216246061179168No Hit
ATGTGACACTTAACGCCCCCCGTGAATACGGAGAGGGGTAGTGCCACTGT597880.16102857039741236No Hit
TTTTTAATGTCTTTAGCGAGACGCTACCATGGCTATCGCTGTAGGTAGCC545140.14682396946953466No Hit
GGCTATCGCTGTAGGTAGCCGGAATTCCATTCCTAGGAGGTTTGACCTGT542620.146145251336462No Hit
CTTTTAGTACCCTTGATAGGGAGAACGAGACCTTCGTCCCCTCCGTTCGC526660.14184670316033518No Hit
CCTTGATCTATCGATGTGACACTTAACGCCCCCCGTGAATACGGAGAGGG523230.1409228923680974No Hit
AGTTAAAACGACCGGGAGTCCAGTTCGAACGATATTTTAAAGAGAATGAG510260.13742964864351312No Hit
CTTAACGCCCCCCGTGAATACGGAGAGGGGTAGTGCCACTGTTTCGTTTT509800.13730575565096811No Hit
GAGAGGGGTAGTGCCACTGTTTCGTTTTGGCCCCAGTCGAGTTAAAACGA508880.1370579696658781No Hit
GGGTAGTGCCACTGTTTCGTTTTGGCCCCAGTCGAGTTAAAACGACCGGG489310.13178713083086546No Hit
GATAAATAGAGAAGGTTTCTTACATGACAAATCCTTGTCATGGGATCCGG478610.12890527209123157No Hit
GCTCAACTTCCTGTCGAGCTAATGCCATTTTTAATGTCTTTAGCGAGACG477850.1287005793209398No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT467340.12586989377387886No Hit
GGTATCAACGCAGAGTACGGGCGACCCCACGATGACCCACTTCGCTTGTA464580.1251265358186088No Hit
ACTTCCTGTCGAGCTAATGCCATTTTTAATGTCTTTAGCGAGACGCTACC463320.12478717675207247No Hit
GATCTATCGATGTGACACTTAACGCCCCCCGTGAATACGGAGAGGGGTAG455680.12272947574545538No Hit
CTATTTATCTGACCGCACCGATCATTCGCCTCCCGTAGCTTAGCGATAGC439870.11847132745820195No Hit
GTTATCTTCAGTCTCACCGTCCGCGTAAACGCGAACGGAGGGGACGAAGG436990.11769564959183318No Hit
GAGTTATCTTCAGTCTCACCGTCCGCGTAAACGCGAACGGAGGGGACGAA421330.1134779011934531No Hit
GTCTCACCGTCCGCGTAAACGCGAACGGAGGGGACGAAGGTCTCGTTCTC419780.11306043567509494No Hit
GGTCAAACCTCCTAGGAATGGAATTCCGGCTACCTACAGCGATAGCCATG417300.11239249084572184No Hit
CCCTATCAAGGGTACTAAAAGCTCGCACAGGTCAAACCTCCTAGGAATGG407650.10979343132820156No Hit
TCTTCAGTCTCACCGTCCGCGTAAACGCGAACGGAGGGGACGAAGGTCTC396710.10684693276637026No Hit
ATATTTTAAAGAGAATGAGTTATCTTCAGTCTCACCGTCCGCGTAAACGC395320.10647256046281035No Hit
GTATAGACCTGAATGATCAATCGATCAACCAGCGTCTGGCTCAGCAGGGC388000.10450104588578979No Hit
CCCCACGATGACCCACTTCGCTTGTAGGCACCTTGATCTATCGATGTGAC373170.10050684353917569No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA874950.063.3600651
GTATCAA2648450.047.1603661
TATGTCC280600.035.9939887
ATGTCCC289250.034.953758
ATATGTC294150.034.6489036
TATTATA314300.034.4431722
TTATATG305400.033.704944
ATTATAT318850.033.1502153
TATATGT316700.032.590835
ATATTAT332600.032.44311
ACTATTG240850.032.0177266
TATCAAC3928850.031.818312
GACTATT247950.031.29917765
TGGGTCG269200.031.06394654
GTCGCTT269050.030.84795257
TTGACTA257750.030.70695363
ATCAACG4136350.030.4547143
GTGAGAT337000.030.4170616
TGACTAT257100.030.26650664
CGCTTTG274600.029.93184959
Produced by FastQC (version 0.11.4)