FastQCFastQC Report
Fri 17 Dec 2021
L-DM5-Next_S13_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM5-Next_S13_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53264740
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1750510.3286433013659693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA525300.037.532361
GTATCAA1117100.036.5148351
TATCAAC1498150.026.7181782
AACGCAG1574050.025.253396
ATCAACG1578600.025.2000713
AGAGTAC1579200.025.1591111
GCAGAGT1576800.025.1342669
CAGAGTA1586500.025.05476810
CAACGCA1608350.024.655465
TCAACGC1630150.024.334264
CGCAGAG1652050.024.0143818
TATTATA151750.023.8599642
ACGCAGA1668600.023.8122467
TTATATG103800.020.8716034
AGTACGG1507000.020.83153213
GAGTACG1546950.020.35657112
TATAATA136900.020.2325672
ATTATAT128450.019.2965623
GTACGGG1635950.019.15771914
TACGGGG1078200.018.74045215
Produced by FastQC (version 0.11.4)