FastQCFastQC Report
Fri 17 Dec 2021
L-DM5-Next_S13_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM5-Next_S13_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53264740
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA518750.038.2958951
GTATCAA1122750.037.4736061
TATCAAC1552850.026.6750142
AACGCAG1655250.025.1707216
ATCAACG1660800.025.0527673
GCAGAGT1661500.025.018079
AGAGTAC1668000.024.96275111
CAGAGTA1674950.024.87811510
CAACGCA1698750.024.5097335
TCAACGC1709250.024.277154
CGCAGAG1729400.024.054118
ACGCAGA1749900.023.8304257
TATTATA156400.022.4455412
TTATATG100450.021.3935834
AGTACGG1572900.021.05687113
GAGTACG1607100.020.64759612
TATAATA132400.020.116972
GTACGGG1699250.019.47672514
TACGGGG1050800.019.38971915
ATTATAT127800.018.7596193
Produced by FastQC (version 0.11.4)