FastQCFastQC Report
Fri 17 Dec 2021
L-DM2-Plus_S2_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM2-Plus_S2_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23482187
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1981220.8437118740260436No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1173240.499629783205457No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG751540.3200468508320797No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT660020.28107262751974504No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGG411670.17531160960433542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGG378520.16119452587614605No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGG352640.15017340590976472No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGG351120.14952610674636055No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGG303600.1292894907957253No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGGGGGGGG298200.12698987534678946No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGGG256940.10941910989806869No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC166100.039.5441671
AGCAGTG251600.034.9751661
TGTGCTC199450.032.5426863
GTGTGCT222650.029.3787752
CAGTGGT314850.027.9981753
AGTGGTA355650.024.8288444
TGGTATC382550.023.1434826
GTGGTAT391700.022.559775
GTGCTCT301150.021.6052134
TCTTCCG623250.020.2906381
GCAGTGG445900.019.7898542
CTTCCGA636550.019.7323282
GGTATCA451350.019.6915827
TTCCGAT638500.019.5248993
GTATCAA460450.019.1502198
TCCGATC667700.018.5874334
CCGATCT661350.018.3357545
TATCAAC501750.017.5464449
TGCTCTT337400.017.2494035
ATCAACG601950.015.6662641
Produced by FastQC (version 0.11.4)