FastQCFastQC Report
Fri 17 Dec 2021
L-DM2-Plus_S2_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM2-Plus_S2_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23482187
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2002640.8528336819734891No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1572510.6696607943714953No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT849310.3616826660992011No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT249480.10624223374083512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAGTG285800.036.7518841
CGACGCT130100.033.251941
CAGTGGT345650.030.7626863
GACGCTC147450.028.6745172
AGTGGTA392500.027.135234
TCTTCCG598850.025.079351
GTGGTAT427450.024.900225
TGGTATC429550.024.8599176
CTTCCGA630050.023.9595362
TTCCGAT637050.023.5863323
GCAGTGG456000.023.3106252
ACGCTCT179850.023.23633
TCCGATC678450.022.2088724
CCGATCT677750.021.668965
GGTATCA515350.020.8202787
GTATCAA524450.020.3521618
CGCTCTT197400.019.9469534
GTGCTCT139000.018.860051
TATCAAC577200.018.5043539
CGATCTA265500.017.2694286
Produced by FastQC (version 0.11.4)