FastQCFastQC Report
Fri 17 Dec 2021
L-DM2-Next_S9_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM2-Next_S9_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46485359
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA745050.047.1744771
GTATCAA1601900.043.0466771
TATCAAC2466950.027.9996622
CAACGCA2481650.027.7478145
AACGCAG2495000.027.6664966
ATCAACG2517650.027.33863
TCAACGC2535700.027.1575434
ACGCAGA2544600.027.138667
AGAGTAC2592400.026.57805411
CAGAGTA2600200.026.54131110
GCAGAGT2617850.026.3425759
CGCAGAG2664000.025.9152978
AGTACGG2522100.023.5586813
GAGTACG2583850.023.08051912
TATTATA150600.021.9725762
GTACGGG2743850.021.58220314
TTATATG124550.020.3736064
ATTATAT141050.020.353023
TACGGGG1267300.020.33207315
ATATTAT138800.020.1912041
Produced by FastQC (version 0.11.4)