FastQCFastQC Report
Fri 17 Dec 2021
L-DM2-Next_S9_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM2-Next_S9_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46485359
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA766600.047.126631
GTATCAA1665550.043.7516441
TATCAAC2635150.027.6915042
CAACGCA2676100.027.53465
AACGCAG2694400.027.3774366
ATCAACG2697450.027.1842333
TCAACGC2712950.027.0198884
ACGCAGA2736950.026.965077
AGAGTAC2788650.026.41130611
CAGAGTA2797450.026.36839910
GCAGAGT2813800.026.182429
CGCAGAG2854200.025.8348
AGTACGG2731700.023.51854313
GAGTACG2778600.023.16288812
GTACGGG2976800.021.5245214
TATTATA153400.021.5155752
TACGGGG1294800.020.9414115
ATTATAT141000.019.8084073
TTATATG126400.019.5212554
TACGGGC674250.019.19397515
Produced by FastQC (version 0.11.4)