FastQCFastQC Report
Fri 17 Dec 2021
L-DM11-Plus_S7_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM11-Plus_S7_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23964610
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGAGCGACCGGCCTAGCCTCTAAAATTACCACAGATAAAGTTCAGTCA881410.3677965132752004No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA722750.3015905537373652No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG707470.29521448502604464No Hit
ATCGAACTACCGGGGCTCTTGTTTTTACGTCCCTTGCTCAGTCAGTTGTT651120.2717006452431314No Hit
ATGTTGTAACCGGGAGCTCTTGGTGCTGTAGTCTCATGCAGCATATTGGC620770.25903613703707257No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT564150.23540963111855356No Hit
GTCTTGCTACCCCGGCTGCAAATGTCCACTTCCAAATATTACAAAAAGAG536800.2239969688636702No Hit
GGACATGTACCGGGAAACATAAAACCTCTCAAGATTGAACCAGGAAGAAA470670.19640211127992485No Hit
ACGGAATTACCGGGGGTAGTCCCAGCTACTCGGGAGGCTGAGGCAGGAGA351570.14670382701825732No Hit
GGTAGTTTACCGGGCATGGTTCCTTGTTTCCTTATATTTGTGATTTTATT337990.14103713767926954No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT328610.1371230326719275No Hit
GTTCGGAGACCGGGATCAAGACCAACTTGGCTAACACAGTGAAACCCCAT293510.12247643504317408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGGG247650.061.236368
GTAACCG104700.057.1892856
CGAACTA99500.056.8935853
GGCCTAG124550.054.1991612
TACGTCC103050.053.59651626
GAACTAC106600.053.15764
GCCTAGC128600.052.90166513
AACTACC108250.052.6335075
TAACCGG115900.051.8084687
ACGTCCC107300.051.4091427
CTAGCCT132600.051.33255415
CGGCCTA132850.050.9483211
ACCGGCC137550.050.496839
GTCAGTT109800.049.699241
AGTTCAG136550.049.37080441
CTACCGG117800.049.160887
ACCGGGG172450.048.819879
ACAGGAC137250.047.9242570
TAGTCAC105800.047.8837655
TGTAACC112600.047.7717635
Produced by FastQC (version 0.11.4)