FastQCFastQC Report
Fri 17 Dec 2021
L-DM11-Next_S12_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM11-Next_S12_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9934136
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATATGTCCCAGTGAGATTGCACCCCGTGTGCTAGAGGCACTTGCC160280.1613426673441958No Hit
ATATAATATCGTCCCCGTAGATGCCTATGGTTCCGGCGTTACCAAAATGG140850.1417838451174818No Hit
GGTTTGACCTGTGCGAGCTTTTAGTACCCTTGATAGGGAGAACGAGACCT119330.12012116604805892No Hit
ATTTTAAAGAGAATGAGTTATCTTCAGTCTCACCGTCCGCGTAAACGCGA104740.10543443335182849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA237350.041.371841
GGTATCA126400.040.0490071
TATTATA50650.032.562982
ATATTAT47800.029.4624671
TATGTCC45550.029.035967
ATTATAT52200.028.3675173
TTATATG49750.027.6453364
TATATGT50550.027.343265
AACGCAG357350.027.2595836
CAACGCA357700.027.215265
TATCAAC364500.027.0531542
ACGCAGA360750.026.9534877
ATGTCCC48800.026.8150128
ATCAACG364500.026.7568553
CAGAGTA374750.026.04267310
GCAGAGT374750.025.9289269
AGAGTAC377300.025.8878211
ATATGTC51950.025.6615666
TCAACGC393400.024.8461444
CGCAGAG394900.024.5868038
Produced by FastQC (version 0.11.4)