FastQCFastQC Report
Fri 17 Dec 2021
L-DM11-Next_S12_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM11-Next_S12_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9934136
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTATATGTCCCAGTGAGATTGCACCCCGTGTGCTAGAGGCACTTGCC154000.15502103051538654No Hit
ATATAATATCGTCCCCGTAGATGCCTATGGTTCCGGCGTTACCAAAATGG137940.13885455161878194No Hit
ATTTTAAAGAGAATGAGTTATCTTCAGTCTCACCGTCCGCGTAAACGCGA106890.1075986879986342No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA237150.041.3228841
GGTATCA128700.040.084331
TATTATA48800.030.0502282
ATATTAT45050.029.4442251
TTATATG47500.027.7043384
ATTATAT49400.027.7015043
TATGTCC46050.027.0537837
TATATGT50800.026.0423075
AACGCAG382900.025.9495146
CAACGCA384100.025.704565
TATCAAC382550.025.6899032
ATCAACG385950.025.5270673
ACGCAGA389550.025.477147
ATGTCCC50500.024.8084328
ATATGTC51850.024.502316
AGAGTAC400650.024.49203511
CAGAGTA401450.024.48693810
GCAGAGT401550.024.4717549
TATAATA49350.024.183592
TCAACGC414900.023.8471184
Produced by FastQC (version 0.11.4)