FastQCFastQC Report
Fri 17 Dec 2021
L-DM10-Plus_S6_R2_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameL-DM10-Plus_S6_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31390933
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1535870.4892718543918398No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG853530.2719033550229297No Hit
ATCACACTTAGACATTGCTTTCCAGCATGGATTCTGACTTAGAGGCCTTC669300.21321443360730946No Hit
ATGGATTCTGACTTAGAGGCCTTCAGTCATAATCCAACAGATGGTAGCTT632450.20147537507088434No Hit
ATTGCTTTCCAGCATGGATTCTGACTTAGAGGCCTTCAGTCATAATCCAA587840.18726426513031644No Hit
ATAATCCAACAGATGGTAGCTTCACGGCATTGTCTTTTCAGACAACCGTA571980.18221185079143715No Hit
CTCCCGTGTATATTTAGCTTTAGAAGAAGTTTATCTCCCACTTAGGGCTG551780.17577687162085945No Hit
CTTTCCAGCATGGATTCTGACTTAGAGGCCTTCAGTCATAATCCAACAGA498160.1586955061195537No Hit
CAAGAGTTCATATCGACGTTGCTTTTTGATCCTTCGATGTCGGCTCTTCC481560.15340735491997004No Hit
ATTTAAGTTAGTACGAGAGGAACCCTTAAATCAGATAATTGGTAAATACG480460.1530569352621663No Hit
TTCACGGCATTGTCTTTTCAGACAACCGTATTTACCAATTATCTGATTTA475910.15160747213215994No Hit
AGAATTGTAATCTAAAGAGTTAACCTAAAGCTAAGCTTCTCGAATAAGTT458000.14590200297646458No Hit
TTTTCGTTTATCATCACACTTAGACATTGCTTTCCAGCATGGATTCTGAC451140.14371665856507038No Hit
CGGCTCTTCCTATCATTGTGAAGCAGAATTCACAACGTGTCGGATTGTTC449550.14321014287788134No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA427870.13630368998589498No Hit
CAACAGATGGTAGCTTCACGGCATTGTCTTTTCAGACAACCGTATTTACC421690.13433496863568853No Hit
AATTTAAGTTAGTACGAGAGGAACCCTTAAATCAGATAATTGGTAAATAC410240.1306874185612769No Hit
AGTAATTTAAGTTAGTACGAGAGGAACCCTTAAATCAGATAATTGGTAAA407260.12973809985195406No Hit
GCTCTTCCTATCATTGTGAAGCAGAATTCACAACGTGTCGGATTGTTCAC401620.12794140269739673No Hit
ATTGTCTTTTCAGACAACCGTATTTACCAATTATCTGATTTAAGGGTTCC400370.12754319854080157No Hit
CTTTTCAGACAACCGTATTTACCAATTATCTGATTTAAGGGTTCCTCTCG399410.1272373777485365No Hit
GTTGCTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGA388360.12371725300423532No Hit
CATCACACTTAGACATTGCTTTCCAGCATGGATTCTGACTTAGAGGCCTT386700.12318843788427696No Hit
TTAGAGGCCTTCAGTCATAATCCAACAGATGGTAGCTTCACGGCATTGTC356080.11343402886432206No Hit
TGTTCACCCGCTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGAC354650.1129784833091772No Hit
CTTCAGTCATAATCCAACAGATGGTAGCTTCACGGCATTGTCTTTTCAGA354320.1128733574118361No Hit
AGTTAGTACGAGAGGAACCCTTAAATCAGATAATTGGTAAATACGGTTGT351300.1119112961695022No Hit
AGTCATAATCCAACAGATGGTAGCTTCACGGCATTGTCTTTTCAGACAAC350980.11180935590541384No Hit
TTGTTCACCCGCTAATAGGGAACGTGAGCTGGGTTTAGACCGTCGTGAGA336750.1072761997867346No Hit
CACTTAGACATTGCTTTCCAGCATGGATTCTGACTTAGAGGCCTTCAGTC334760.10664225876943512No Hit
GTTTTACCCTACTGATGAAACGATGTTGCGACAGTAATTTAAGTTAGTAC334180.10645749204077495No Hit
CTTTTTGATCCTTCGATGTCGGCTCTTCCTATCATTGTGAAGCAGAATTC330200.105189610006176No Hit
ACTTAGACATTGCTTTCCAGCATGGATTCTGACTTAGAGGCCTTCAGTCA329980.10511952607461524No Hit
AGCATGGATTCTGACTTAGAGGCCTTCAGTCATAATCCAACAGATGGTAG325500.10369236237737821No Hit
CTTAGAGGCCTTCAGTCATAATCCAACAGATGGTAGCTTCACGGCATTGT320480.10209317448449207No Hit
TTAGACATTGCTTTCCAGCATGGATTCTGACTTAGAGGCCTTCAGTCATA315830.10061185502195809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC188100.050.005551
AGCAGTG375350.046.954871
GCAGTGG405200.043.614682
CAGTGGT450600.039.2730143
TGTGCTC249550.037.0909653
GTGTGCT257550.036.260212
AGTGGTA595250.029.8931354
GTGGTAT636600.027.9324535
TGGTATC660350.027.000066
GGTATCA726650.024.543927
GTGCTCT400250.023.2340814
CGCTCTT101150.022.8581351
GTATCAA780300.022.8203328
AAGCAGT104050.022.5240651
TATCAAC883450.020.2551489
ATCAACG1004150.017.74146710
TCAACGC1028750.017.29846811
TGCTCTT521300.016.8236375
CGATCTC218400.016.647836
TTCCGAT852950.016.4049473
Produced by FastQC (version 0.11.4)